Use of morphological traits and microsatellite markers to characterize the Tunisian cultivated and wild figs (Ficus carica L.)

被引:14
作者
Ahmed, Ben Abdelkrim [1 ,2 ,3 ]
Ghada, Baraket [1 ]
Laila, Essalouh [4 ,5 ]
Hafid, Achtak [6 ,7 ]
Bouchaib, Khadari [5 ]
Amel, Salhi-Hannachi [1 ]
机构
[1] Univ Tunis El Manar, Fac Sci Tunis, Lab Genet Mol Immunol & Biotechnol, Tunis 2092, Tunisia
[2] Univ Rouen, CNRS, Lab Polymeres Biopolymeres Surfaces, UMR 6270, Mont St Aignan, France
[3] PISSARO Prote Platform, Mont St Aignan, France
[4] Montpellier SupAgro, AGAP, UMR 1334, Montpellier, France
[5] INRA, CBNMed, UMR AGAP, Montpellier, France
[6] Cadi Ayyad Univ, Fac Sci & Tech, Lab Biotechnol Valorisat & Protect Agroresources, Marrakech, Morocco
[7] Cadi Ayyad Univ, Fac Polydisciplinaire Safi, Dept Biol, Safi 46000, Morocco
关键词
Wild figs; Ficus carica L; Identification key; Molecular marker; Morphological traits; SSRs; Tunisia; COMMON FIG; GENETIC DIVERSITY; LOCI;
D O I
10.1016/j.bse.2015.01.026
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We used 8 morphological traits and 17 simple sequence repeats loci to characterize 71 cultivated and wild Tunisian fig trees (Ficus carcia L.). Significant morphological differences were inferred from leaf traits. The statistical analysis showed two major fig groups that indicated a common morphological basis. A total of 74 SSR alleles was revealed, defining 63 unique multilocus genotypes indicating a substantial genetic diversity. Based on multilocus SSR genotypes an identification key was established using MFC30, MFC3, MFC11 and MFC19 loci to identify figs. Analysis of variance components of linkage disequilibrium shown that among 136 pairs of loci, 32 present a significant gametic disequilibrium. The parameter D'(2)(IS) (0.1284) was greater than D'(2)(ST) (0.0079), a pointer of close to zero variance in total simple, and consequently the more pronounced independence of the 17 SSR loci. The majority of Ohta's variance components of linkage disequilibrium followed a pattern caused by genetic drift or a non-systematic disequilibrium profiles and natural selection occurs only for LMFC24-MFC8 pair loci in cultivated figs. Our results suggest that the morphological and SSR markers are suitable to characterize figs and should be recommended in conservation management strategy. (C) 2015 Elsevier Ltd. All rights reserved.
引用
收藏
页码:209 / 219
页数:11
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