Response surface optimization for recovery of polyphenols and carotenoids from leaves of Centella asiatica using an ethanol-based solvent system

被引:36
作者
Gunathilake, K. D. P. P. [1 ,2 ]
Ranaweera, K. K. D. S. [2 ]
Rupasinghe, H. P., V [3 ]
机构
[1] Wayamba Univ Sri Lanka, Fac Livestock Fisheries & Nutr, Dept Food Sci & Technol, Makandura, Gonawila, Sri Lanka
[2] Univ Sri Jayewardenepura, Dept Food Sci & Technol, Fac Appl Sci, Nugegoda, Sri Lanka
[3] Dalhousie Univ, Dept Plant Food & Environm Sci, Fac Agr, Truro, NS, Canada
基金
美国国家科学基金会;
关键词
carotenoids; Centella asiatica leaves; phenolics; response surface methodology; ULTRASONIC-ASSISTED EXTRACTION; ANTIOXIDANT CAPACITY; PHENOLIC-COMPOUNDS; LEAF; L;
D O I
10.1002/fsn3.832
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Response surface methodology has been used to optimize the extraction conditions for total phenolics and carotenoids from leaves of Centella asiatica. Solvent concentration (30%-100%), extraction temperature (30-60 degrees C), and extraction time (3090 min) were used as the independent variables. A second-order polynomial model produced a satisfactory fitting of the experimental data with regard to total phenolics (R-2 = 84.75%, p < 0.004) and carotenoid (R-2 = 78.74, p < 0.019) contents. The optimum extraction conditions of ethanol concentration, extraction temperature, and extraction time for phenolics were 6.1%, 70.2 degrees C, and 110.5 min and for carotenoids, the optimum parameters were 100%, 70.2 degrees C, and 110.5 min, respectively. The optimal predicted contents for total phenolics (9.03 mg Gallic Acid Equivalent (GAE)/g DW) and carotenoid (8.74 mg/g DW) values in the extracts were agreed with the experimental values obtained with optimum extraction conditions for each response, and also they possess significantly higher total antioxidant capacity.
引用
收藏
页码:528 / 536
页数:9
相关论文
共 34 条
[21]  
Rahman M., 2013, J Pharm Phytochem, V1, P27
[22]   Accelerated solvent extraction: A technique for sample preparation [J].
Richter, BE ;
Jones, BA ;
Ezzell, JL ;
Porter, NL ;
Avdalovic, N ;
Pohl, C .
ANALYTICAL CHEMISTRY, 1996, 68 (06) :1033-1039
[23]   Optimization of olive leaf extract obtained by ultrasound-assisted extraction with response surface methodology [J].
Sahin, Selin ;
Samli, Ruya .
ULTRASONICS SONOCHEMISTRY, 2013, 20 (01) :595-602
[24]  
SIES H, 1995, AM J CLIN NUTR, V62, P1315
[25]   Optimisation of the adsorption of polyphenols from Inga edulis leaves on macroporous resins using an experimental design methodology [J].
Silva, E. M. ;
Pompeu, D. R. ;
Larondelle, Y. ;
Rogez, H. .
SEPARATION AND PURIFICATION TECHNOLOGY, 2007, 53 (03) :274-280
[26]  
Singleton VL, 1999, METHOD ENZYMOL, V299, P152
[27]   Optimized microwave-assisted extraction of total phenolics (TP) from Ipomoea batatas leaves and its antioxidant activity [J].
Song, Jiangfeng ;
Li, Dajing ;
Liu, Chunquan ;
Zhang, Ying .
INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES, 2011, 12 (03) :282-287
[28]  
Srivastava Ritu, 1997, Journal of Medicinal and Aromatic Plant Sciences, V19, P1049
[29]   Antioxidant activity of carotenoids [J].
Stahl, Wilhelm ;
Sies, Helmut .
MOLECULAR ASPECTS OF MEDICINE, 2003, 24 (06) :345-351
[30]   Optimization of ultrasonic-assisted extraction of natural antioxidants from rice bran using response surface methodology [J].
Tabaraki, Reza ;
Nateghi, Ashraf .
ULTRASONICS SONOCHEMISTRY, 2011, 18 (06) :1279-1286