Removal of oil and grease using immobilized triacylglycerin lipase

被引：19

作者：

Dumore, Nilesh S. ^{[1
]}

Mukhopadhyay, Mausumi ^{[1
]}

机构：

[1] Sardar Vallabhbhai Natl Inst Technol, Dept Chem Engn, Surat 395007, India

来源：

INTERNATIONAL BIODETERIORATION & BIODEGRADATION | 2012年 / 68卷

关键词：

Immobilization; Adsorption; Aspergillus niger lipase; Chitosan; Celite; Cellulose acetate; SLAUGHTERHOUSE WASTE-WATER; ACTIVATED CARBON; PRETREATMENT; CHITOSAN; WASTEWATERS; HYDROLYSIS; ADSORPTION; DEGRADATION; REDUCTION; EMULSIONS;

D O I：

10.1016/j.ibiod.2011.12.005

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In the present work, Triacylglycerin lipase (Aspergillus niger lipase) was immobilized on chitosan, celite and cellulose acetate polymer structure by adsorption. The concentration of protein in lipase solution before and after immobilization was determined by using the Lowry's method and analyzed using Uv-Spectrophotometer. After 12 h, the protein yield percentage of lipase on chitosan, celite and cellulose acetate support were 93.7%, 90.2% and 87.3%. Surface characterization of immobilized lipase was determined by using optical microscopy. Chitosan immobilized lipase bead (most suitable) from A. niger bead was used for removal of oil and grease (O&G) from synthetic oily water with bead concentration of 0.1-0.3 g/l. Approximately 48% of the O&G was hydrolyzed by 0.3 g/l bead of chitosan immobilized lipase from A. niger. In addition, about 47% reduction in COD was estimated after degradation of synthetic oily water with chitosan immobilized lipase. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：65 / 70

页数：6

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