Size control of giant unilamellar vesicles prepared from inverted emulsion droplets

被引：77

作者：

Nishimura, Kazuya ^{[1
]}

Suzuki, Hiroaki ^{[1
,2
]}

Toyota, Taro ^{[3
]}

Yomo, Tetsuya ^{[1
,2
,4
]}

机构：

[1] Osaka Univ, Dept Bioinformat Engn, Grad Sch Informat Sci & Technol, Suita, Osaka 5650871, Japan

[2] Japan Sci & Technol Agcy, Suita, Osaka 5650871, Japan

[3] Univ Tokyo, Grad Sch Arts & Sci, Meguro Ku, Tokyo 1538902, Japan

[4] Osaka Univ, Grad Sch Frontier Biosci, Dept Frontier Biosci, Suita, Osaka 5650871, Japan

来源：

JOURNAL OF COLLOID AND INTERFACE SCIENCE | 2012年 / 376卷

关键词：

Giant lipid vesicles; Microfluidics; Water-in-oil emulsion; Micro-reaction compartment; LIPID VESICLES; IN-OIL; ACTIN-FILAMENTS; RNA-SYNTHESIS; LIPOSOMES; PERMEABILITY; MONODISPERSE; MEMBRANE; MICROFLUIDICS; MICRODROPLETS;

D O I：

10.1016/j.jcis.2012.02.029

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The production of giant lipid vesicles with controlled size and structure will be an important technology in the design of quantitative biological assays in cell-mimetic microcompartments. For establishing size control of giant vesicles, we investigated the vesicle formation process, in which inverted emulsion droplets are transformed into giant unilamellar vesicles (GUVs) when they pass through an oil/water interface. The relationship between the size of the template emulsion and the converted GUVs was studied using inverted emulsion droplets with a narrow size distribution, which were prepared by microfluidics. We successfully found an appropriate centrifugal acceleration condition to obtain GUVs that had a desired size and narrow-enough size distribution with an improved yield so that emulsion droplets can become the template for GUVs. Crown Copyright (C) 2012 Published by Elsevier Inc. All rights reserved.

引用

页码：119 / 125

页数：7

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