Rehmannia inhibits adipocyte differentiation and adipogenesis

被引：21

作者：

Jiang, Lin ^{[1
]}

Zhang, Nai-xian ^{[1
]}

Mo, Wei ^{[1
]}

Wan, Rui ^{[1
]}

Ma, Chun-gu ^{[1
]}

Li, Xi ^{[1
]}

Gu, Yin-liang ^{[1
]}

Yang, Xin-ying ^{[1
]}

Tang, Qi-qun ^{[1
]}

Song, Hou-yan ^{[1
]}

机构：

[1] Fudan Univ, Minist Educ, Key Lab Mol Med, Shanghai 200032, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2008年 / 371卷 / 02期

关键词：

Rehmannia glutinosa; 3T3-L1; adipocyte differentiation; adipogenesis;

D O I：

10.1016/j.bbrc.2008.03.129

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Rehmannia glutinosa, a Traditional Chinese Medicine (TCM), has been used to increase physical strength. Here, we report that Rehmannia glutinosa extract (RE) inhibits adipocyte differentiation and adipogenesis. RE impairs differentiation of 3T3-L1 preadipocytes in a dose-dependent manner. At the molecular level, treatment with RE inhibits expression of the key adipocyte differentiation regulator C/EBP beta, as well as C/EBP alpha and the terminal marker protein 422/aP2, during differentiation of preadipocytes into adipocytes. Additionally, RE inhibits the mitotic clonal expansion (MCE) process of adipocyte differentiation, and RE prevents localization of C/EBP beta to the centromeres. RE also prevents high fat diet (HFD) induced weight gain and adiposity in rats. Taken together, our results indicate that Rehmannia glutinosa extract inhibits preadipocyte differentiation and adipogenesis in cultured cells and in rodent models,of obesity. (C) 2008 Published by Elsevier Inc.

引用

页码：185 / 190

页数：6

共 22 条

[11] A 30-KDA ALTERNATIVE TRANSLATION PRODUCT OF THE CCAAT ENHANCER-BINDING PROTEIN-ALPHA MESSAGE - TRANSCRIPTIONAL ACTIVATOR LACKING ANTIMITOTIC ACTIVITY [J].

LIN, FT ;

MACDOUGALD, OA ;

DIEHL, AM ;

LANE, MD .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (20) :9606-9610

[12] ANTISENSE CCAAT ENHANCER-BINDING PROTEIN RNA SUPPRESSES COORDINATE GENE-EXPRESSION AND TRIGLYCERIDE ACCUMULATION DURING DIFFERENTIATION OF 3T3-L1 PREADIPOCYTES [J].

LIN, FT ;

LANE, MD .

GENES & DEVELOPMENT, 1992, 6 (04) :533-544

[13] TRANSCRIPTIONAL REGULATION OF GENE-EXPRESSION DURING ADIPOCYTE DIFFERENTIATION [J].

MACDOUGALD, OA ;

LANE, MD .

ANNUAL REVIEW OF BIOCHEMISTRY, 1995, 64 :345-373

[14] The disease burden associated with overweight and obesity [J].

Must, A ;

Spadano, J ;

Coakley, EH ;

Field, AE ;

Colditz, G ;

Dietz, WH .

JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 1999, 282 (16) :1523-1529

[15] 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitor impairs cell differentiation in cultured adipogenic cells (3T3-L1) [J].

Nishio, E ;

Tomiyama, K ;

Nakata, H ;

Watanabe, Y .

EUROPEAN JOURNAL OF PHARMACOLOGY, 1996, 301 (1-3) :203-206

[16] CCAAT ENHANCER-BINDING PROTEIN MESSENGER-RNA IS TRANSLATED INTO MULTIPLE PROTEINS WITH DIFFERENT TRANSCRIPTION ACTIVATION POTENTIALS [J].

OSSIPOW, V ;

DESCOMBES, P ;

SCHIBLER, U .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (17) :8219-8223

[17] THE CCAAT ENHANCER BINDING-PROTEIN AND ITS ROLE IN ADIPOCYTE DIFFERENTIATION - EVIDENCE FOR DIRECT INVOLVEMENT IN TERMINAL ADIPOCYTE DEVELOPMENT [J].

SAMUELSSON, L ;

STROMBERG, K ;

VIKMAN, K ;

BJURSELL, G ;

ENERBACK, S .

EMBO JOURNAL, 1991, 10 (12) :3787-3793

[18] Obesity and the regulation of energy balance [J].

Spiegelman, BM ;

Flier, JS .

CELL, 2001, 104 (04) :531-543

[19] CCAAT/enhancer-binding protein β is required for mitotic clonal expansion during adipogenesis [J].

Tang, QQ ;

Otto, TC ;

Lane, MD .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (03) :850-855

[20] Mitotic clonal expansion: A synchronous process required for adipogenesis [J].

Tang, QQ ;

Otto, TC ;

Lane, MD .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (01) :44-49

← 1 2 3 →