Oxidant and antioxidant parameters in patients with Brucella canis

Objectives: The aim of this study was to evaluate the oxidative status in patient with Brucella canis (B. canis) and healthy controls by measurement of total antioxidant capacity (TAC) and total oxidant capacity (TOS), and calculation of oxidative stress index (OSI). Design and methods: Six patients with B. canis and 6 healthy control subjects were included in this study. Diagnosis of B. canis was made by rapid slide agglutination test (RSAT) and Modified Plate Agglutination Test (MPAT). The serum samples of B. canis positive patients and controls were used for measurement of TAC and TOS levels. Results: The TOS and OSI index were significantly higher in brucellosis positive patients than control group (p<0.001 and p<0.0001, respectively), while TAC level was found similar in patient and control groups. Conclusion: Findings of this study have shown that B. canis generate low TOS and OSI index in man compared with smooth Brucella species infection. This difference may help to provide understanding about the pathogenesis of B. cools infection in man. (C) 2012 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.