Isolation of Lysozyme from Chicken Egg White Using Polyacrylamide-based Cation-exchange Cryogel

被引：39
作者：
Yan Luding [1 ]
Shen Shaochuan [1 ]
Yun Junxian [1 ]
Yao Kejian [1 ]
机构：
[1] Zhejiang Univ Technol, Coll Chem Engn & Mat Sci, State Key Lab Breeding Base Green Chem Synth Tech, Hangzhou 310032, Zhejiang, Peoples R China
来源：
CHINESE JOURNAL OF CHEMICAL ENGINEERING | 2011年 / 19卷 / 05期
基金：
中国国家自然科学基金;
关键词：
cation-exchange cryogel; lysozyme; isolation; sequential elution; chicken egg white; PURIFICATION;
D O I：
10.1016/S1004-9541(11)60068-2
中图分类号：
TQ [化学工业];
学科分类号：
0817 ;
摘要：
An effective cation-exchange chromatographic method for lysozyme isolation from chicken egg white is presented, using supermacroporous cryogel grafted with sulfo functional groups. The chromatographic processes were carried out by one-step and sequential elution, respectively. Sodium phosphate buffer (pH 7.8) containing different concentrations of NaCl is used as elution agent. The corresponding breakthrough characteristics and elution behaviors in the cryogel bed were investigated and analyzed. Purity of lysozyme in the elution effluent was assayed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The maximum purity of the obtained lysozyme was about 96%, and the cryogel is demonstrated as a potential separation medium for purification of high-purity lysozyme from chicken egg white.
引用
收藏
页码：876 / 880
页数：5
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