Establishment and characterization of a cisplatin-resistant cell line (IGSK-1) from a poorly differentiated gastric adenocarcinoma

被引：8

作者：

Ohi, Satoshi ^{[1
]}

Takahashi, Naoto ^{[2
]}

Ninomiya, Kouzou ^{[1
]}

Nakajima, Masako ^{[1
]}

Hashimoto, Hisashi ^{[1
]}

Tachibana, Toshiaki ^{[1
]}

Yanaga, Katsuhiko ^{[2
]}

Ishikawa, Hiroshi ^{[1
]}

机构：

[1] Jikei Univ, Sch Med, Dept Anat 2, Tokyo, Japan

[2] Jikei Univ, Sch Med, Dept Surg, Tokyo, Japan

来源：

HUMAN CELL | 2007年 / 20卷 / 01期

关键词：

cell line; cisplatin; gastric cell; gastrin or somatostatin secretion; poorly differentiated adenocarcinoma;

D O I：

10.1111/j.1749-0774.2007.00024.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We successfully established a spontaneously cisplatin-resistant tumor cell line (designated as IGSK- 1) derived from original gastric carcinoma. The patient was a 75-year-old Japanese woman. The histopathological diagnosis was gastric poorly differentiated adenocarcinoma accompanied with metastatic foci in lymph nodes, pT3, N2 MO, stage IIIB. The IGSK-1 cells grew as adhesive and monolayered cultures on the bottom of dishes. The susceptibility of the IGSK-1 cells to anti-cancer drugs was examined using oxygen electrode apparatus (Daikin, Tsukuba, JPN), and the results suggested TXL was effective, and CDDP, CPT-II and 5-FU were not effective. Gastrin and somatostatin secretions were confirmed by immunohistochemical staining and also radioimmunoassay. Immunohistochemistry and radioimmunoassay for serotonin suggested the IGSK-1 cells might incorporate serotonin from the growth media. Spontaneously cisplatin-resistant gastric carcinoma cell line secreted gastrin and somatostatin is very important material for chemotherapy.

引用

页码：15 / 22

页数：8

共 15 条

[1]

AKAGI T, 1976, ACTA MED OKAYAMA, V30, P215

[2] CHARACTERISTICS OF 3 HUMAN GASTRIC-CANCER CELL-LINES, NU-GC-2, NU-GC-3 AND NU-GC-4 [J].

AKIYAMA, S ;

AMO, H ;

WATANABE, T ;

MATSUYAMA, M ;

SAKAMOTO, J ;

IMAIZUMI, M ;

ICHIHASHI, H ;

KONDO, T ;

TAKAGI, H .

JAPANESE JOURNAL OF SURGERY, 1988, 18 (04) :438-446

[3]

ARIMURA A, 1991, J HUM CELL, V4, P67

[4]

KAMEYA T, 1975, CANCER RES, V35, P2025

[5]

Ohi Satoshi, 2005, Hum Cell, V18, P35

[6]

PARK JG, 1990, CANCER RES, V50, P2773

[7]

SEKIGUCHI M, 1978, JPN J EXP MED, V48, P61

[8]

Terano A, 1989, Hum Cell, V2, P307

[9]

TERANO A, 1989, Gastroenterologia Japonica, V24, P219

[10] ESTABLISHMENT OF A CSF-PRODUCING CELL-LINE FROM A HUMAN GASTRIC-CARCINOMA AND CHARACTERISTICS OF THE CSF PRODUCED BY THAT CELL-LINE [J].

UEDA, M ;

FUJIWARA, Y ;

MURAKAMI, A ;

TAKAHASHI, M ;

MIZOGUCHI, H .

INTERNATIONAL JOURNAL OF CELL CLONING, 1987, 5 (04) :322-334

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