Algae Polar Lipids Characterized by Online Liquid Chromatography Coupled with Hybrid Linear Quadrupole Ion Trap/Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

被引：39

作者：

He, Huan ^{[1
]}

Rodgers, Ryan P. ^{[1
,2
]}

Marshall, Alan G. ^{[1
,2
]}

Hsu, Chang Samuel ^{[3
]}

机构：

[1] Florida State Univ, Natl High Magnet Field Lab, Tallahassee, FL 32310 USA

[2] Florida State Univ, Dept Chem & Biochem, Tallahassee, FL 32306 USA

[3] Florida State Univ, Future Fuels Inst, Tallahassee, FL 32310 USA

来源：

ENERGY & FUELS | 2011年 / 25卷 / 10期

基金：

美国国家科学基金会;

关键词：

BIODIESEL;

D O I：

10.1021/ef201061J

中图分类号：

TE [石油、天然气工业]; TK [能源与动力工程];

学科分类号：

0807 ; 0820 ;

摘要：

We report the first application of online LC-MS (liquid chromatography-mass spectrometry) characterization of algae polar lipids by nanoscale high-performance liquid chromatography followed by electrospray ionization and mass analysis with a linear ion trap (LTQ) coupled with 14.5 T Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS). Ultrahigh FT-ICR mass resolution provides highly accurate mass measurement and resolves monoisotopic peaks from interfering components for unique determination of lipid elemental compositions. We establish the polar lipid profile of fatty acids, glycolipids, phospholipids, and betaine lipids for a green algae, Nannochloropsis oculata, which is highly prized for its oils suitable for biodiesel production. Lipid headgroup and fatty acid identification is based on accurate mass measured by the FT-ICR MS and collision-induced dissociation (CID) MS/MS in the LTQ, Unequivocal lipid composition is further confirmed from isotopic fine structure at baseline resolution-achievable only with ultrahigh resolution FT-ICR MS.

引用

页码：4770 / 4775

页数：6

共 25 条

[1]

[Anonymous], Algae Oil Yields

[2] Effect of temperature and nitrogen concentration on the growth and lipid content of Nannochloropsis oculata and Chlorella vulgaris for biodiesel production [J].

Converti, Attilio ;

Casazza, Alessandro A. ;

Ortiz, Erika Y. ;

Perego, Patrizia ;

Del Borghi, Marco .

CHEMICAL ENGINEERING AND PROCESSING-PROCESS INTENSIFICATION, 2009, 48 (06) :1146-1151

[3] STORED WAVE-FORM INVERSE FOURIER-TRANSFORM AXIAL EXCITATION/EJECTION FOR QUADRUPOLE ION-TRAP MASS-SPECTROMETRY [J].

GUAN, SH ;

MARSHALL, AG .

ANALYTICAL CHEMISTRY, 1993, 65 (09) :1288-1294

[4] Method for lipidomic analysis: p53 expression modulation of sulfatide, ganglioside, and phospholipid composition of U87 MG glioblastoma cells [J].

He, Huan ;

Conrad, Charles A. ;

Nilsson, Carol L. ;

Ji, Yongjie ;

Schaub, Tanner M. ;

Marshall, Alan G. ;

Emmett, Mark R. .

ANALYTICAL CHEMISTRY, 2007, 79 (22) :8423-8430

[5] Petroleomics: advanced molecular probe for petroleum heavy ends [J].

Hsu, Chang S. ;

Hendrickson, Christopher L. ;

Rodgers, Ryan P. ;

McKenna, Amy M. ;

Marshall, Alan G. .

JOURNAL OF MASS SPECTROMETRY, 2011, 46 (04) :337-343

[6]

HSU CS, 2011, 59 AM SOC MASS SPECT

[7] SPACE-CHARGE EFFECTS IN FOURIER-TRANSFORM MASS-SPECTROMETRY - MASS CALIBRATION [J].

LEDFORD, EB ;

REMPEL, DL ;

GROSS, ML .

ANALYTICAL CHEMISTRY, 1984, 56 (14) :2744-2748

[8] Isolation and characterization of a xanthophyll aberrant mutant of the green alga Nannochloropsis oculata [J].

Lee, Mi-Young ;

Min, Byung-Sul ;

Chang, Chung-Soon ;

Jin, EonSeon .

MARINE BIOTECHNOLOGY, 2006, 8 (03) :238-245

[9]

Marshall AG, 1998, MASS SPECTROM REV, V17, P1, DOI 10.1002/(SICI)1098-2787(1998)17:1<1::AID-MAS1>3.0.CO

[10]

2-K

← 1 2 3 →