Hyaluronic acid/chitosan nanoparticles for delivery of curcuminoid and its in vitro evaluation in glioma cells

被引：100

作者：

Yang, Liu ^{[1
]}

Gao, Shiya ^{[1
]}

Asghar, Sajid ^{[1
,2
]}

Liu, Guihua ^{[1
]}

Song, Jue ^{[1
]}

Wang, Xuan ^{[1
]}

Ping, Qineng ^{[1
]}

Zhang, Can ^{[1
]}

Xiao, Yanyu ^{[1
]}

机构：

[1] China Pharmaceut Univ, Dept Pharmaceut, State Key Lab Nat Med, Nanjing 210009, Jiangsu, Peoples R China

[2] Govt Coll Univ Faisalabad, Coll Pharm, Faisalabad, Pakistan

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2015年 / 72卷

关键词：

Curcuminoid; Polyelectrolyte complex nanoparticles; Brain gliomas; POLYELECTROLYTE COMPLEX; CHITOSAN NANOPARTICLES; ANTICANCER; SYSTEMS; PACLITAXEL; DENSITY;

D O I：

10.1016/j.ijbiomac.2014.10.039

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The aim of this work was to evaluate the potential of polyelectrolyte complex nanoparticles (PENPs) based on hyaluronic acid/chitosan (HA/CS) as carriers for water-insoluble curcuminoid (CUR) and explore in vitro performance against brain glioma cells. PENPs were observed to be affected by the order of addition, mass ratios and initial concentrations of the HA/CS, pH and ionic strength. PENPs remained stable over a temperature range of 5-55 (C. CUR was successfully encapsulated into the PENPs. CUR-PENPs showed spherical shape with a mean diameter of 207 nm and positive charge of 25.37 mV. High encapsulation efficiency (89.9%) and drug loading (6.5%) was achieved. Drug release studies revealed initial burst release of drug from the PENPs up to 4 h followed by sustained release pattern. DSC thermograms and XRD patterns showed that CUR was encapsulated inside the PENPs in a molecular or amorphous state. Compared with CUR-solution. CUR-PENPs showed stronger dose dependent cytotoxicity against C6 glioma cells and higher performance in uptake efficiency in C6 cells. Cellular uptake of CUR-PENPs was found to be governed by multi-mechanism in C6 cells, involving active endocytosis, macropinocytosis, clathrin-, caveolae-, and CD44-mediated endocytosis. In conclusion, CUR-PENPs might be a promising carrier for therapy of brain gliomas. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：1391 / 1401

页数：11

共 42 条

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