Graphene-DNAzyme Based Biosensor for Amplified Fluorescence "Turn-On" Detection of Pb2+ with a High Selectivity

被引：348

作者：

Zhao, Xu-Hua ^{[1
]}

Kong, Rong-Mei ^{[1
]}

Zhang, Xiao-Bing ^{[1
]}

Meng, Hong-Min ^{[1
]}

Liu, Wei-Na ^{[1
]}

Tan, Weihong ^{[1
]}

Shen, Guo-Li ^{[1
]}

Yu, Ru-Qin ^{[1
]}

机构：

[1] Hunan Univ, State Key Lab Chemo Biosensing & Chemometr, Coll Chem & Chem Engn, Changsha 410082, Hunan, Peoples R China

来源：

ANALYTICAL CHEMISTRY | 2011年 / 83卷 / 13期

基金：

中国国家自然科学基金;

关键词：

IN-VITRO SELECTION; LEAD; OXIDE; SENSOR; ION; SPECTROMETRY; CADMIUM; SAMPLES; COPPER;

D O I：

10.1021/ac200843x

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

On the basis of the remarkable difference in affinity of graphene (GO) with ssDNA containing a different number of bases in length, we for the first time report a GO-DNAzyme based biosensor for amplified fluorescence "turn-on" detection of Pb2+. A FAM-labeled DNAzyme-substrate hybrid acted as both a molecular recognition module and signal reporter and GO as a superquencher. By taking advantage of the super fluorescence quenching efficiency of GO, our proposed biosensor exhibits a high sensitivity toward the target with a detection limit of 300 pM for Pb2+, which is lower than previously reported for catalytic beacons. Moreover, with the choice of a classic Pb2+-dependent GR-5 DNAzyme instead of 8-17 DNAzyme as the catalytic unit, the newly designed sensing system also shows an obviously improved selectivity than previously reported methods. Moreover, the sensing system was used for the determination of Pb2+ in river water samples with satisfying results.

引用

页码：5062 / 5066

页数：5

共 39 条

[21] Determination of trace copper, lead, cadmium, and iron in environmental and biological samples by flame atomic absorption spectrometry coupled to flow injection on-line coprecipitation preconcentration using DDTC-nickel as coprecipitate carrier [J].