Monolithic silica-based capillary reversed-phase liquid chromatography/electrospray mass spectrometry for plant metabolomics

被引：197
作者：
Tolstikov, VV
Lommen, A
Nakanishi, K
Tanaka, N
Fiehn, O [1 ]
机构：
[1] Max Planck Inst Mol Plant Physiol, Dept Lothar Willmitzer, D-14424 Potsdam, Germany
[2] RIKILT, Inst Food Safety, NL-6700 AE Wageningen, Netherlands
[3] Plant Res Int, NL-6700 AA Wageningen, Netherlands
[4] Kyoto Univ, Dept Chem Mat, PRESTO, JST,Sakyo Ku, Kyoto 6068501, Japan
[5] Kyoto Inst Technol, Dept Polymer Sci & Engn, Sakyo Ku, Kyoto 6068585, Japan
来源：
ANALYTICAL CHEMISTRY | 2003年 / 75卷 / 23期
关键词：
D O I：
10.1021/ac034716z
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Application of C18 monolithic silica capillary columns in HPLC coupled to ion trap mass spectrometry detection was studied for probing the metabolome of the model plant Arabidopsis thaliana. It could be shown that the use of a long capillary column is an easy and effective approach to reduce ionization suppression by enhanced chromatographic resolution. Several hundred peaks could be detected using a 90-cm capillary column for LC separation and a noise reduction and automatic peak alignment software, which outperformed manual inspection or commercially available mass spectral deconvolution software.
引用
收藏
页码：6737 / 6740
页数：4
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