Important roles played by TGF-β member of Bmdpp and Bmdaw in BmNPV infection

被引：8

作者：

Hu, Xiaolong ^{[1
,2
]}

Jiang, Yue ^{[1
]}

Gong, Yongchang ^{[1
]}

Zhu, Min ^{[1
]}

Zhu, Liyuan ^{[1
]}

Chen, Fei ^{[1
]}

Liang, Zi ^{[1
]}

Kuang, Sulan ^{[1
]}

Zar, Mian Sahib ^{[1
]}

Kumar, Dhiraj ^{[1
]}

Cao, Guangli ^{[1
,2
]}

Xue, Renyu ^{[1
,2
]}

Gong, Chengliang ^{[1
,2
]}

机构：

[1] Soochow Univ, Sch Biol & Basic Med Sci, Suzhou 215123, Peoples R China

[2] Soochow Univ, Natl Engn Lab Modern Silk, Suzhou 215123, Peoples R China

来源：

MOLECULAR IMMUNOLOGY | 2016年 / 73卷

基金：

中国国家自然科学基金; 中国博士后科学基金;

关键词：

Bombyx mori; TGF-β decapentaplegic; dawdle; BmNPV; GROWTH-FACTOR-BETA; JAK/STAT PATHWAY; IMMUNE-RESPONSES; BOMBYX-MORI; DROSOPHILA; HOMEOSTASIS; GENE; DPP; RESISTANCE; REGULATORS;

D O I：

10.1016/j.molimm.2016.04.004

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Transforming growth factor (TGF)-beta superfamily members inhibit Bombyx mori nucleohedrovirus (BmNPV) multiplication in silkworm are not determined. In this study, we first found that BmNPV RNA transcription and protein expression level were regulated by TGF-beta members, Decapentaplegic (Bmdpp) and Dawdle (Bmdaw) in the domesticated silkworm, B. mori and silkworm ovary-derived cells. Furthermore, subcellular localization showed that Bmdpp and Bmdaw were mainly presented in cytomembrane of the cultured BmN cells. Tissues expression pattern analysis found that the highest expression levels of Bmdpp and Bmdaw genes were in the hemocyte of fifth instar larvae. During the immune response, the expression level of Bmdpp gene was elevated and Bmdaw gene was declined in BmNPV infected BmN cells and silkworm. The multiplication of BmNPV was inhibited by overexpression of Bmdpp and Bmdaw genes in BmN cells. RNA interference experiments found that the multiplication of BmNPV was raised with specific siRNAs of Bmdpp and Bmdaw genes in BmN cells. The antiviral immune pathways were not significantly regulated by the TGF-beta superfamily members. Taken together, these findings provided a clue to understand the function of Bmdpp and Bmdaw gene in response to the BmNPV infection in silkworm. (c) 2016 Elsevier Ltd. All rights reserved.

引用

页码：122 / 129

页数：8

共 29 条

[1] The long and short of antiviral defense: small RNA-based immunity in insects [J].

Bronkhorst, Alfred W. ;

van Rij, Ronald P. .

CURRENT OPINION IN VIROLOGY, 2014, 7 :19-28

[2] Multiple TGF-β Superfamily Signals Modulate the Adult Drosophila Immune Response [J].

Clark, Rebecca I. ;

Woodcock, Katie J. ;

Geissmann, Frederic ;

Trouillet, Celine ;

Dionne, Marc S. .

CURRENT BIOLOGY, 2011, 21 (19) :1672-1677

[3] TGF-β signaling in tumor suppression and cancer progression [J].

Derynck, R ;

Akhurst, RJ ;

Balmain, A .

NATURE GENETICS, 2001, 29 (02) :117-129

[4] Activin signaling functions upstream of Gbb to regulate synaptic growth at the Drosophila neuromuscular junction [J].

Ellis, J. E. ;

Parker, L. ;

Cho, J. ;

Arora, K. .

DEVELOPMENTAL BIOLOGY, 2010, 342 (02) :121-133

[5] Specificity and versatility in TGF-β signaling through Smads [J].

Feng, XH ;

Derynck, R .

ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, 2005, 21 :659-693

[6] Systemic Activin signaling independently regulates sugar homeostasis, cellular metabolism, and pH balance in Drosophila melanogaster [J].

Ghosh, Arpan C. ;

O'Connor, Michael B. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2014, 111 (15) :5729-5734

[7] A screen identifying genes responsive to Dpp and Wg signaling in the Drosophila developing wing [J].

Hadar, Neuman ;

Yaron, Suissa ;

Oren, Ziv ;

Elly, Ordan ;

Itamar, Weisman ;

Johnathan, Grossman ;

Tama, Dinur ;

Offer, Gerlitz .

GENE, 2012, 494 (01) :65-72

[8] Activation and function of TGFβ signalling during Drosophila wing development and its interactions with the BMP pathway [J].

Hevia, Covadonga F. ;

de Celis, Jose F. .

DEVELOPMENTAL BIOLOGY, 2013, 377 (01) :138-153

[9] The JAK/STAT pathway in model organisms: Emerging roles in cell movement [J].

Hou, SX ;

Zheng, ZY ;

Chen, X ;

Perrimon, N .

DEVELOPMENTAL CELL, 2002, 3 (06) :765-778

[10] Insect Cytokine Paralytic Peptide (PP) Induces Cellular and Humoral Immune Responses in the Silkworm Bombyx mori [J].

Ishii, Kenichi ;

Hamamoto, Hiroshi ;

Kamimura, Manabu ;

Nakamura, Yuki ;

Noda, Hiroaki ;

Imamura, Katsutoshi ;

Mita, Kazuei ;

Sekimizu, Kazuhisa .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2010, 285 (37) :28635-28642

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