Interferon status and white blood cells during infection with African swine fever virus in vivo

被引：45

作者：

Karalyan, Z. ^{[1
]}

Zakaryan, H. ^{[1
]}

Sargsyan, Kh ^{[2
]}

Voskanyan, H. ^{[1
]}

Arzumanyan, H. ^{[1
]}

Avagyan, H. ^{[1
]}

Karalova, E. ^{[1
]}

机构：

[1] Inst Mol Biol NAS, Cell Biol Lab, Yerevan 0014, Armenia

[2] Sci Ctr Stock Breading & Vet RA, Yerevan 0071, Armenia

来源：

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY | 2012年 / 145卷 / 1-2期

关键词：

African swine fever virus; Atypical lymphocyte; Infection; Interferon; BONE-MARROW; MACROPHAGES; INHIBITION; RESPONSES; MONOCYTES; GENES; GAMMA;

D O I：

10.1016/j.vetimm.2011.12.013

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

African swine fever virus (ASFV) is the causative agent of African swine fever that is the significant disease of domestic pigs, with high rates of mortality. ASFV is double-stranded DNA virus whose genes encode some proteins that are implicated in the suppression of host immune response. In this study, we have modeled in vivo infection of ASFV for determination of interferon (IFN) status in infected pigs. We measured the level of IFN-alpha, -beta and -gamma by enzyme-linked immunosorbent assay and showed that the level of IFN-alpha sharply decreased during infection. Unlike IFN-alpha, the level of IFN-beta and -gamma increased from the 2nd and 4th days post-infection, respectively. Also, we analyzed the population dynamics of peripheral white blood cells of infected pigs due to their important role in host immune system. We showed that the atypical lymphocytes appeared after short time of infection and this result is in accordance with our previous study done in vitro. At the last day of infection about 50% of the total white blood cells were destroyed, and the remaining cells were represented mainly by small-sized lymphocytes, reactive lymphocytes and lymphoblasts. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：551 / 555

页数：5

共 20 条

[11] Cytopathogenesis and inhibition of host gene expression by RNA viruses [J].