Genetic structure of Varroa destructor populations infesting Apis mellifera colonies in Argentina

被引：21
作者：
Maggi, M. [1 ,2 ]
Medici, S. [1 ,2 ]
Quintana, S. [3 ]
Ruffinengo, S. [4 ]
Marcangeli, J. [1 ]
Gimenez Martinez, P. [1 ]
Fuselli, S. [1 ,5 ]
Eguaras, M. [1 ,2 ]
机构：
[1] Univ Nacl Mar del Plata, Fac Ciencias Exactas & Nat, Lab Artropodos, RA-7600 Mar Del Plata, Argentina
[2] Consejo Nacl Invest Cient & Tecn, CONICET, Buenos Aires, DF, Argentina
[3] Fares Taie Inst Anal, RA-7600 Mar Del Plata, Argentina
[4] Univ Nacl Mar del Plata, Fac Ciencias Agrarias, Balcarce, Argentina
[5] CIC, RA-1900 La Plata, Buenos Aires, Argentina
来源：
EXPERIMENTAL AND APPLIED ACAROLOGY | 2012年 / 56卷 / 04期
关键词：
Varroa destructor; DNA; Haplotypes; Argentina; HONEY-BEES; AFRICANIZED BEES; ACARI VARROIDAE; JACOBSONI; MITES; IDENTIFICATION; RESISTANCE; GENOTYPES; PARASITE; CERANA;
D O I：
10.1007/s10493-012-9526-0
中图分类号：
Q96 [昆虫学];
学科分类号：
摘要：
Although mitochondrial DNA mapping of Varroa destructor revealed the presence of several haplotypes, only two of them (Korean and Japanese haplotypes) were capable to infest Apis mellifera populations. Even though the Korean haplotype is the only one that has been reported in Argentina, these conclusions were based on mites sampled in apiaries from a specific geographical place (Buenos Aires province). To study mites from several sites of Argentina could reveal the presence of the Japanese genotype, especially considering sites near to Brazil, where Japanese haplotype was already detected. The aim of this work was to study the genetic structure of V. destructor populations from apiaries located in various provinces of Argentina, in order to determine the presence of different haplotypes. The study was carried out between January 2006 and December 2009. Phoretic adult Varroa mites were collected from honey bee workers sampled from colonies of A. mellifera located in Entre Rios, Buenos Aires, Corrientes, Rio Negro, Santa Cruz and Neuqu,n provinces. Twenty female mites from each sampling site were used to carry out the genetic analysis. For DNA extraction a nondestructive method was used. DNA sequences were compared to Korean haplotype (AF106899) and Japanese haplotype (AF106897). All DNA sequences obtained from mite populations sampled in Argentina, share 98% of similitude with Korean Haplotype (AF106899). Taking into account these results, we are able to conclude that Korean haplotype is cosmopolite in Argentina.
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页码：309 / 318
页数：10
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