High-reproducible flow cytometric endothelial progenitor cell determination in human peripheral blood as CD34+/CD144+/CD3-lymphocyte sub-population

被引：25

作者：

Redondo, Santiago ^{[1
]}

Hristov, Mihail ^{[2
,3
]}

Gordillo-Moscoso, Antonio A. ^{[1
]}

Ruiz, Emilio ^{[1
]}

Weber, Christian ^{[2
]}

Tejerina, Teresa ^{[1
]}

机构：

[1] Univ Complutense, Dept Pharm, Sch Med, E-28040 Madrid, Spain

[2] RWTH Aachen Univ Hosp, Inst Mol Cardiovasc Res, Aachen, Germany

[3] RWTH Aachen Univ Hosp, Interdisciplinary Ctr Clin Res BIOMAT, Aachen, Germany

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 2008年 / 335卷 / 1-2期

关键词：

flow cytometry; endothelial progenitor cells; markers;

D O I：

10.1016/j.jim.2008.02.011

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Although determination of circulating endothelial progenitor cell (EPC) in peripheral blood by flow cytometry is an emerging marker for cardiovascular medicine, a common standardized protocol is still not available, due to the low numbers achieved in peripheral blood. In the present paper we describe a novel technique for EPC quantification as CD34+/CD144+/CD3-cells within the lymphocyte gate, which increases the percentages of EPC positivity described before and also offers high intra-assay reproducibility. These improvements are based on a gating strategy for big-sized lymphocytes, smooth fixation and cytometric clearance of CD3+ lymphocytes (T-cells). This last procedure is able to increase intra-assay Pearson's correlation from 0.8517 to 0.8908. Therefore, the technical setting described here offers a high-performance and clinically oriented EPC determination strategy in human peripheral blood. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：21 / 27

页数：7

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