Purification and characterization of four antibacterial peptides from protamex hydrolysate of Atlantic mackerel (Scomber scombrus) by-products

被引：107
作者：
Ennaas, Nadia [1 ]
Hammami, Riadh [1 ]
Beaulieu, Lucie [1 ,2 ]
Fliss, Ismail [1 ]
机构：
[1] Univ Laval, STEM Dairy Res Ctr, Inst Nutr & Funct Foods, Quebec City, PQ G1V 0A6, Canada
[2] UQAR, Dept Biol Chem & Geog, Rimouski, PQ G51 3A1, Canada
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2015年 / 462卷 / 03期
关键词：
Antimicrobial peptide; Mackerel by-products; Amino acids; Purification; Listeria; Escherichia coli; SPONTANEOUSLY HYPERTENSIVE-RATS; ENZYME INHIBITORY PEPTIDE; FRAME PROTEIN HYDROLYSATE; ANTIMICROBIAL PEPTIDE; MACADAMIA-INTEGRIFOLIA; ANTIOXIDANT PROPERTIES; SKIN; COLLAGEN; REGIONS; TILAPIA;
D O I：
10.1016/j.bbrc.2015.04.091
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Proteins from fish by-product sources are valuable source of bioactive peptides and show promise as functional foods ingredients. The objective of the present study was to isolate and characterize antibacterial peptides from protamex hydrolysates of Atlantic mackerel (Scomber scombrus) by-products. Four sequences SIFIQRFTT (P4), RKSGDPLGR (P8.1), AKPGDGAGSGPR (P8.2) and GLPGPLGPAGPK (P11) were identified in peptide fractions separated using RP-HPLC. At 200 mu g mL(-1), while peptides P8.1, P8.2 and P11 exhibited partial inhibition, P4 totally inhibited tested Gram-positive (Listeria innocua) and Gram-negative (Escherichia coli) bacterial strains. These results suggest that the protein hydrolysate derived from mackerel by-products could be used as an antimicrobial ingredient in both functional food and nutraceutical applications. (C) 2015 Elsevier Inc. All rights reserved.
引用
收藏
页码：195 / 200
页数：6
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