Secreted microRNA data from the parasitic filarial nematode Acanthocheilonema viteae

被引：4

作者：

Soichot, Julien ^{[1
]}

Endriss, Yvette ^{[2
]}

Marti, Hanspeter ^{[2
]}

Tritten, Lucienne ^{[1
]}

机构：

[1] Univ Zurich, Inst Parasitol, Winterthurerstr 266a, CH-8057 Zurich, Switzerland

[2] Swiss Trop & Publ Hlth Inst, Socinstr 57, CH-4051 Basel, Switzerland

来源：

DATA IN BRIEF | 2020年 / 29卷

基金：

瑞士国家科学基金会;

关键词：

microRNA; Excretory-secretory; Extracellular vesicle; Filarial nematode; Parasite;

D O I：

10.1016/j.dib.2020.105334

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

microRNAs (miRNAs) are an abundant class of non-coding RNA species with important regulatory roles in gene expression at the posttranscriptional level. The helminth Acanthocheilonema viteae serves as model organism for research on parasitic filarial nematodes. Total RNA secreted or excreted in vitro by 1500 adult female and male A. viteae over 3 weeks was isolated from culture media previously processed by differential ultracentrifugation. miRNA sequencing revealed the presence of 360 unique miRNA candidates released by adult A. viteae in vitro. Among them, 74 highconfidence unique miRNAs, as well as several potential novel miRNA candidates were discovered. A large proportion of the sequenced miRNA candidates appeared differentially expressed between the male and female samples based on normalized copy count. The presence of extracellular vesicles, often rich in miRNAs, could not be confirmed unambiguously by transmission electron microscopy. (c) 2020 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http:// creativecommons.org/ licenses/by-nc-nd/4.0/).

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