A single exposure to a sublethal pediocin concentration initiates a resistance-associated temporal cell envelope and general stress response in Listeria monocytogenes

被引:20
作者
Laursen, Martin F. [1 ,2 ]
Bahl, Martin I. [2 ]
Licht, Tine R. [2 ]
Gram, Lone [1 ]
Knudsen, Gitte M. [1 ]
机构
[1] Tech Univ Denmark, Dept Syst Biol, DK-2800 Lyngby, Denmark
[2] Tech Univ Denmark, Natl Food Inst, DK-2800 Lyngby, Denmark
关键词
MANNOSE PHOSPHOTRANSFERASE SYSTEM; CLASS-IIA BACTERIOCINS; PLANTARUM WHE 92; EGD-E; MESENTERICIN Y105; CROSS-PROTECTION; PCR DATA; VIRULENCE; ANTIBIOTICS; EXPRESSION;
D O I
10.1111/1462-2920.12534
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Listeria monocytogenes can cause the potentially fatal food-borne disease listeriosis, and the use of bacteriocin-producing lactic acid bacteria to control L. monocytogenes holds great promise. However, the development of bacteriocin resistance is a potential challenge, and the purpose of this study was to determine if exposure to sublethal concentrations of pediocin-containing Lactobacillus plantarumWHE 92 supernatant could prime L. monocytogenes for resistance. By transcriptomic analysis, we found two, 55 and 539 genes differentially expressed after 10, 60 and 180min of exposure to L. plantarumWHE 92 supernatant as compared with control exposures. We observed temporal expression changes in genes regulated by the two component system LisRK and the alternative sigma factors SigB and SigL. Additionally, several genes involved in bacteriocin resistance were induced. lisR, sigB and sigL mutants were all more resistant than wild types to L. plantarumWHE 92 supernatant. Conclusively, LisRK, SigB and SigL regulation and genes associated with resistance are involved in the temporal adaptive response to pediocin, and all three regulatory systems affect pediocin resistance. Thus, a single exposure to a sublethal pediocin concentration initiates a response pointing to resistance, and indicates that further research exploring the link between adaptive responses and resistance is needed.
引用
收藏
页码:1134 / 1151
页数:18
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