Quantitative determination of vasicine and vasicinone in Adhatoda vasica by high performance capillary electrophoresis

被引：20

作者：

Avula, B. ^{[1
,4
]}

Begum, S. ^{[1
,4
]}

Ahmed, S. ^{[1
,4
]}

Choudhary, M. I. ^{[3
,4
]}

Khan, I. A. ^{[1
,2
,4
]}

机构：

[1] Univ Mississippi, Sch Pharm, Natl Ctr Nat Products Res, University, MS 38677 USA

[2] Univ Mississippi, Sch Pharm, Res Inst Pharmaceut Sci, Dept Pharmacognosy, University, MS 38677 USA

[3] Univ Karachi, HEJ Res Inst Chem, Karachi 32, Pakistan

[4] Univ Karachi, Int Ctr Chem & Biol Sci, Karachi 32, Pakistan

来源：

PHARMAZIE | 2008年 / 63卷 / 01期

关键词：

D O I：

10.1691/ph.2008.7175

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

A new method of capillary electrophoresis was developed for the quantitative determination of vasicine and vasicinone from Adhatoda vasica (L.) Nees. The electrophoretic separation was performed using a 47 cm x 50 mu m ID (38.5 cm effective length) fused silica capillary. The samples were injected by pressure for 3 s at 50 mbar and the running voltage was 19 kV at the injector end of the capillary. The capillary temperature was maintained at 40 degrees C. The separation of the two alkaloids has been achieved within 11 min with good repeatability. The method was validated in terms of reproducibility, linearity, accuracy and applied for the quantitative determination of vasicine and vasicinone in A. vasica plant samples/extracts. Parameters affecting the resolution such as pH, temperature, organic modifier, buffer concentration and capillary dimensions were reported.

引用

页码：20 / 22

页数：3

共 12 条

[1]

Atal C, 1980, Chemistry and pharmacology of vasicine

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