A Comparative Analysis of the Structure and Biological Properties of Films and Microfibrous Scaffolds Based on Silk Fibroin

被引：6
作者：
Safonova, Liubov [1 ]
Bobrova, Maria [1 ]
Efimov, Anton [1 ]
Lyundup, Alexey [2 ]
Agapova, Olga [1 ]
Agapov, Igor [1 ]
机构：
[1] Minist Hlth Russian Federat, Academician VI Shumakov Natl Med Res Ctr Transpla, Schukinskaya Ul 1, Moscow 123182, Russia
[2] Peoples Friendship Univ Russia, Educ Resource Ctr Cellular Technol, Miklukho Maklaya Str 6, Moscow 117198, Russia
来源：
PHARMACEUTICS | 2021年 / 13卷 / 10期
关键词：
silk fibroin films; biocompatible polymers; electrospinning; scanning probe nanotomography; full-thickness skin wound; SCANNING PROBE NANOTOMOGRAPHY; REGENERATED SILK; MECHANICAL-PROPERTIES; IN-VITRO; TISSUE; FABRICATION; GELATIN; CHITOSAN; NANOSTRUCTURE; PROLIFERATION;
D O I：
10.3390/pharmaceutics13101561
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
A comparative analysis of the structure and biological properties of silk fibroin constructions was performed. Three groups of constructions were obtained: films obtained by casting an aqueous solution of silk fibroin and electrospun microfibrous scaffolds based on silk fibroin, with the addition of 30% gelatin per total protein weight. The internal structures of the films and single fibers of the microfibrous scaffolds consisted of densely packed globule structures; the surface area to volume ratios and volume porosities of the microfibrous scaffolds were calculated. All constructions were non-toxic for cells and provide high levels of adhesion and proliferation. The high regenerative potential of the constructions was demonstrated in a rat full-thickness skin wound healing model. The constructions accelerated healing by an average of 15 days and can be considered to be promising constructions for various tasks of tissue engineering and regenerative medicine.
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页数：16
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