Effect of quercetin and its metabolites isorhamnetin and quercetin-3-glucuronide on inflammatory gene expression: role of miR-155

被引：213

作者：

Boesch-Saadatmandi, Christine ^{[1
]}

Loboda, Agnieszka ^{[2
]}

Wagner, Anika E. ^{[1
]}

Stachurska, Anna ^{[2
]}

Jozkowicz, Alicja ^{[2
]}

Dulak, Jozef ^{[2
]}

Doering, Frank ^{[1
]}

Wolffram, Siegfried ^{[3
]}

Rimbach, Gerald ^{[1
]}

机构：

[1] Univ Kiel, Inst Human Nutr & Food Sci, D-24098 Kiel, Germany

[2] Jagiellonian Univ, Dept Med Biotechnol, Fac Biochem Biophys & Biotechnol, PL-30387 Krakow, Poland

[3] Univ Kiel, Inst Anim Nutr & Physiol, D-24098 Kiel, Germany

来源：

JOURNAL OF NUTRITIONAL BIOCHEMISTRY | 2011年 / 22卷 / 03期

基金：

英国惠康基金;

关键词：

Quercetin; Macrophage; Inflammation; Flavonoid metabolism; MicroRNA; ADHESION MOLECULE EXPRESSION; KAPPA-B ACTIVITY; FLAVONOID QUERCETIN; PLATELET-AGGREGATION; DIETARY FLAVONOIDS; ENDOTHELIAL-CELLS; APOE GENOTYPE; IN-VITRO; PLASMA; PATHWAY;

D O I：

10.1016/j.jnutbio.2010.02.008

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In the present study the effect of quercetin and its major metabolites quercetin-3-glucuronide (Q3G) and isorhamnetin on inflammatory gene expression was determined in murine RAW264.7 macrophages stimulated with lipopolysaccharide. Quercetin and isorhamnetin but not Q3G significantly decreased mRNA and protein levels of tumor necrosis factor alpha. Furthermore a significant decrease in mRNA levels of interleukin 13, interleukin 6, macrophage inflammatory protein 1 alpha and inducible nitric oxide synthase was evident in response to the quercetin treatment. However Q3G did not affect inflammatory gene expression. Anti-inflammatory properties of quercetin and isorhamnetin were accompanied by an increase in heme oxygenase 1 protein levels, a downstream target of the transcription factor Nrf2, known to antagonize chronic inflammation. Furthermore, proinflammatory microRNA-155 was down-regulated by quercetin and isorhamnetin but not by Q3G. Finally, anti-inflammatory properties of quercetin were confirmed in vivo in mice fed quercetin-enriched diets (0.1 mg quercetin/g diet) over 6 weeks. (C) 2011 Elsevier Inc. All rights reserved.

引用

页码：293 / 299

页数：7

共 47 条

[21]

Manach C, 2005, AM J CLIN NUTR, V81, p230S, DOI 10.1093/ajcn/81.1.230S

[22] QUERCETIN METABOLITES IN PLASMA OF RATS FED DIETS CONTAINING RUTIN OR QUERCETIN [J].

MANACH, C ;

MORAND, C ;

TEXIER, O ;

FAVIER, ML ;

AGULLO, G ;

DEMIGNE, C ;

REGERAT, F ;

REMESY, C .

JOURNAL OF NUTRITION, 1995, 125 (07) :1911-1922

[23] Determination of flavonol metabolites in plasma and tissues of rats by HPLC-radiocounting and tandem mass spectrometry following oral ingestion of [2-14C]quercetin-4' glucoside [J].

Mullen, W ;

Graf, BA ;

Caldwell, ST ;

Hartley, RC ;

Duthie, GG ;

Edwards, CA ;

Lean, MEJ ;

Crozier, A .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2002, 50 (23) :6902-6909

[24] Antioxidative flavonoid quercetin: implication of its intestinal absorption and metabolism [J].

Murota, K ;

Terao, J .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2003, 417 (01) :12-17

[25] The flavonoid quercetin inhibits proinflammatory cytokine (tumor necrosis factor alpha) gene expression in normal peripheral blood mononuclear cells via modulation of the NF-κβ system [J].

Nair, MP ;

Mahajan, S ;

Reynolds, JL ;

Aalinkeel, R ;

Nair, H ;

Schwartz, SA ;

Kandaswami, C .

CLINICAL AND VACCINE IMMUNOLOGY, 2006, 13 (03) :319-328

[26] Regulatory potential for concerted modulation of Nrf2-and Nfkb1-mediated gene expression in inflammation and carcinogenesis [J].

Nair, S. ;

Doh, S. T. ;

Chan, J. Y. ;

Kong, A-N ;

Cai, L. .

BRITISH JOURNAL OF CANCER, 2008, 99 (12) :2070-2082

[27] Sustained expression of microRNA-155 in hematopoietic stem cells causes a myeloproliferative disorder [J].

O'Connell, Ryan M. ;

Rao, Dinesh S. ;

Chaudhuri, Aadel A. ;

Boldin, Mark P. ;

Taganov, Konstantin D. ;

Nicoll, John ;

Paquette, Ronald L. ;

Baltimore, David .

JOURNAL OF EXPERIMENTAL MEDICINE, 2008, 205 (03) :585-594

[28] MicroRNA-155 is induced during the macrophage inflammatory response [J].

O'Connell, Ryan M. ;

Taganov, Konstantin D. ;

Boldin, Mark P. ;

Cheng, Genhong ;

Baltimore, David .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (05) :1604-1609

[29] Metabolism of quercetin-7-and quercetin-3-glucuronides by an in vitro hepatic model:: the role of human β-glucuronidase, sulfotransferase, catechol-O-methyltransferase and multi-resistant protein 2 (MRP2) in flavonoid metabolism [J].

O'Leary, KA ;

Day, AJ ;

Needs, PW ;

Mellon, FA ;

O'Brien, NM ;

Williamson, G .

BIOCHEMICAL PHARMACOLOGY, 2003, 65 (03) :479-491

[30] Flavonoid glucuronides are substrates for human liver β-glucuronidase [J].

O'Leary, KA ;

Day, AJ ;

Needs, PW ;

Sly, WS ;

O'Brien, NM ;

Williamson, G .

FEBS LETTERS, 2001, 503 (01) :103-106

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