Supercritical fluid chromatography tandem-column method development in pharmaceutical sciences for a mixture of four stereoisomers

被引：33

作者：

Barnhart, WW ^{[1
]}

Gahm, KH ^{[1
]}

Thomas, S ^{[1
]}

Notari, S ^{[1
]}

Semin, D ^{[1
]}

Cheetham, J ^{[1
]}

机构：

[1] Amgen Inc, Discovery Anal Sci, Mol Struct, Thousand Oaks, CA 91320 USA

来源：

JOURNAL OF SEPARATION SCIENCE | 2005年 / 28卷 / 07期

关键词：

supercritical fluid chromatography; tandem-column; diastereomer;

D O I：

10.1002/jssc.200500005

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A tandem-column method using Chiralpak AD-H and Chiralcel OD-H columns was achieved for baseline separation of a mixture of chiral pharmaceutical compounds (i.e., four stereoisomers) via supercritical fluid chromatography (SFC) with a mobile phase consisting of 90% liquid carbon dioxide and 10% ethanol: isopropanol (50:50 v/v). On the contrary, this mixture (mixture A) could not be baseline separated by SFC conditions explored with individual Chiralpak AD-H and Chiralcel OD-H columns. The effects of various mobile phases on elution order, capacity factor, selectivity, and resolution were determined with mixture A on the individual aforementioned columns to develop the tandem-column method.

引用

页码：619 / 626

页数：8

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