Protective Effect of ALA in Crushed Optic Nerve Cat Retinal Ganglion Cells Using a New Marker RBPMS

被引：10
|
作者：
Wang, Yanling [1 ,2 ,3 ]
Wang, Wenyao [1 ,2 ,3 ]
Liu, Jessica [4 ,5 ,6 ]
Huang, Xin [1 ,2 ,3 ]
Liu, Ruixing [1 ,2 ,3 ]
Xia, Huika [1 ,2 ,3 ]
Brecha, Nicholas C. [4 ,5 ,6 ]
Pu, Mingliang [1 ,2 ,3 ]
Gao, Jie [1 ,2 ,3 ]
机构：
[1] Peking Univ, Sch Basic Med Sci, Dept Anat, Beijing, Peoples R China
[2] Peking Univ, Key Lab Machine Percept, Minist Educ, Beijing, Peoples R China
[3] Peking Univ, Key Lab Visual Impairment & Restorat, Minist Educ, Beijing, Peoples R China
[4] Univ Calif Los Angeles, Dept Neurobiol & Mol Cellular & Integrat Physiol, Los Angeles, CA USA
[5] Univ Calif Los Angeles, Jules Stein Eye Inst, Los Angeles, CA 90024 USA
[6] Univ Calif Los Angeles, Coll Life Sci, Los Angeles, CA USA
来源：
PLOS ONE | 2016年 / 11卷 / 08期
基金：
美国国家科学基金会;
关键词：
RNA-BINDING PROTEIN; LIPOIC ACID; QUANTITATIVE-ANALYSIS; ADULT-RATS; GLAUCOMA; PRESSURE; SURVIVAL; GENE; EXPRESSION; FIBERS;
D O I：
10.1371/journal.pone.0160309
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
In this study we first sought to determine whether RNA-binding protein with multiple splicing (RBPMS) can serve as a specific marker for cat retina ganglion cells (RGCs) using retrograde labeling and immunohistochemistry staining. RBPM was then used as an RGC marker to study RGC survival after optic nerve crush (ONC) and alpha-lipoic acid (ALA) treatment in cats. ALA treatment yielded a peak density of RBPMS-alpha cells within the peak isodensity zone (>60/mm(2)) which did not differ from ONC retinas. The area within the zone was significantly enlarged (control: 2.3%, ONC: 0.06%, ONC+ALA: 0.1%). As for the 10-21/mm(2) zone, ALA treatment resulted in a significant increase in area (control: 34.5%, ONC: 12.1%, ONC+ALA: 35.9%). ALA can alleviate crush-induced RGC injury.
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页数：16
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