Effects of hyperoxia on transdifferentiation of primary cultured typeII alveolar epithelial cells from premature rats

被引:16
作者
Lu, Hong-Yan [1 ,3 ]
Shao, Gen-Bao [2 ]
Li, Wen-Bin [3 ]
Wang, Hong [3 ]
机构
[1] Jiangsu Univ, Affiliated Hosp, Dept Pediat, Zhenjiang 212001, Peoples R China
[2] Jiangsu Univ, Dept Cell Biol, Sch Med, Zhenjiang 212013, Peoples R China
[3] Huazhong Univ Sci & Technol, Dept Pediat, Tongji Med Coll, Wuhan 430030, Peoples R China
关键词
TypeII alveolar epithelial cells; Proliferation; Transdifferentiation; Hyperoxia; Premature; DNA-DAMAGE; GROWTH ARREST; EXPRESSION; MICE; INHIBITION; EXPOSURE; PROTEIN;
D O I
10.1007/s11626-010-9360-9
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Hyperoxia exposure is a significant risk factor for the impaired alveolarization characteristic of bronchopulmonary dysplasia. Type II alveolar epithelial cells (AECIIs) may serve as "alveolar stem cells" to transdifferentiate into type I alveolar epithelial cells (AECIs). Here, we show that hyperoxia is capable of inducing transdifferentiation of AECIIs in premature rats in vitro. Hyperoxia-induced transdifferentiation was characterized by typical morphological changes, inhibition of cellular proliferation, decline in expression rate of Ki67, accumulation of cells in the G(1) phase of the cell cycle, increased expression of AECI-specific protein aquaporin 5, and decreased expression of AECII-associated protein surfactant protein C. These results suggest that hyperoxia may induce transdifferentiation of AECIIs into AECIs and the transdifferentiation may be responsible for repairing early lung injury.
引用
收藏
页码:64 / 72
页数:9
相关论文
共 29 条
[1]  
ADAMSON IYR, 1974, LAB INVEST, V30, P35
[2]   DNA damage induced by hyperoxia - Quantitation and correlation with lung injury [J].
Barker, George F. ;
Manzo, Nicholas D. ;
Cotich, Kara L. ;
Shone, Robin K. ;
Waxman, Aaron B. .
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 2006, 35 (03) :277-288
[3]   Pulmonary epithelial stem cells [J].
Bishop, AE .
CELL PROLIFERATION, 2004, 37 (01) :89-96
[4]   Keratinocyte growth factor modulates alveolar epithelial cell phenotype in vitro:: Expression of aquaporin 5 [J].
Borok, Z ;
Lubman, RL ;
Danto, SI ;
Zhang, XL ;
Zabski, SM ;
King, LS ;
Lee, DM ;
Agre, P ;
Crandall, ED .
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 1998, 18 (04) :554-561
[5]   Control mechanisms of lung alveolar development and their disorders in bronchopulmonary dysplasia [J].
Bourbon, J ;
Boucherat, O ;
Chailley-Heu, B ;
Delacourt, C .
PEDIATRIC RESEARCH, 2005, 57 (05) :38R-46R
[6]   Caveolin-1 expression and caveolae biogenesis during cell transdifferentiation in lung alveolar epithelial primary cultures [J].
Campbell, L ;
Hollins, AJ ;
Al-Eid, A ;
Newman, GR ;
von Ruhland, C ;
Gumbleton, M .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1999, 262 (03) :744-751
[7]   Enhanced binding of Sp1/Sp3 transcription factors mediates the hyperoxia-induced increased expression of the lung type I cell gene T1α [J].
Cao, YX ;
Ramirez, MI ;
Williams, MC .
JOURNAL OF CELLULAR BIOCHEMISTRY, 2003, 89 (05) :887-901
[8]   Developmental differences in the responses of IL-6 and IL-13 transgenic mice exposed to hyperoxia [J].
Choo-Wing, Rayman ;
Nedrelow, Jonathan H. ;
Homer, Robert J. ;
Elias, Jack A. ;
Bhandari, Vineet .
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY, 2007, 293 (01) :L142-L150
[9]  
CRAPO JD, 1982, AM REV RESPIR DIS, V126, P332
[10]   REVERSIBLE TRANSDIFFERENTIATION OF ALVEOLAR EPITHELIAL-CELLS [J].
DANTO, SI ;
SHANNON, JM ;
BOROK, Z ;
ZABSKI, SM ;
CRANDALL, ED .
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 1995, 12 (05) :497-502