共 58 条
Use of MALDI Mass Spectrometry Imaging to Identify Proteomic Signatures in Aortic Aneurysms after Endovascular Repair
被引:6
作者:
Buerger, Matthias
[1
,2
,3
]
Klein, Oliver
[4
]
Kapahnke, Sebastian
[1
,2
,3
]
Mueller, Verena
[1
,2
,3
]
Frese, Jan Paul
[1
,2
,3
]
Omran, Safwan
[1
,2
,3
]
Greiner, Andreas
[1
,2
,3
]
Sommerfeld, Manuela
[5
,6
,7
]
Kaschina, Elena
[5
,6
,7
]
Jannasch, Anett
[8
]
Dittfeld, Claudia
[8
]
Mahlmann, Adrian
[9
]
Hinterseher, Irene
[1
,2
,3
,10
]
机构:
[1] Charite Univ Med Berlin, Berlin Inst Hlth, Vasc Surg Clin, Hindenburgdamm 30, D-12203 Berlin, Germany
[2] Free Univ Berlin, Hindenburgdamm 30, D-12203 Berlin, Germany
[3] Humboldt Univ, Hindenburgdamm 30, D-12203 Berlin, Germany
[4] Charite Univ Med Berlin, BIH Ctr Regenerat Therapies BCRT, Berlin Inst Hlth, Augustenburger Pl 1, D-13353 Berlin, Germany
[5] Charite Univ Med Berlin, Inst Pharmacol, Ctr Cardiovasc Res CCR, Hessische Str 3-4, D-10115 Berlin, Germany
[6] Free Univ Berlin, Hessische Str 3-4, D-10115 Berlin, Germany
[7] Humboldt Univ, Hessische Str 3-4, D-10115 Berlin, Germany
[8] Tech Univ Dresden, Med Fac Carl Gustav Carus Dresden, Dept Cardiac Surg, Herzzentrum Dresden, D-01307 Dresden, Germany
[9] Tech Univ, Univ Hosp Carl Gustav Carus, Univ Ctr Vasc Med, Dept Med,Sect Angiol, D-01307 Dresden, Germany
[10] Med Hsch Brandenburg Theordor Fontane, D-16816 Neuruppin, Germany
来源:
关键词:
aortic aneurysm;
EVAR;
endoleak;
proteomic signature;
MALDI-MSI;
ELECTRON-TRANSFER FLAVOPROTEIN;
EVAR TRIAL 1;
SPATIAL SEGMENTATION;
MUSCLE;
OUTCOMES;
MATRIX;
GENERATION;
PEPTIDE;
D O I:
10.3390/biomedicines9091088
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
Endovascular repair (EVAR) has become the standard procedure in treating thoracic (TAA) or abdominal aortic aneurysms (AAA). Not entirely free of complications, a persisting perfusion of the aneurysm after EVAR, called Endoleak (EL), leads to reintervention and risk of secondary rupture. How the aortic wall responds to the implantation of a stentgraft and EL is mostly uncertain. We present a pilot study to identify peptide signatures and gain new insights in pathophysiological alterations of the aortic wall after EVAR using matrix-assisted laser desorption or ionization mass spectrometry imaging (MALDI-MSI). In course of or accompanying an open aortic repair, tissue sections from 15 patients (TAA = 5, AAA = 5, EVAR = 5) were collected. Regions of interest (tunica media and tunica adventitia) were defined and univariate (receiver operating characteristic analysis) statistical analysis for subgroup comparison was used. This proof-of-concept study demonstrates that MALDI-MSI is feasible to identify discriminatory peptide signatures separating TAA, AAA and EVAR. Decreased intensity distributions for actin, tropomyosin, and troponin after EVAR suggest impaired contractility in vascular smooth muscle cells. Furthermore, inability to provide energy caused by impaired respiratory chain function and continuous degradation of extracellular matrix components (collagen) might support aortic wall destabilization. In case of EL after EVAR, this mechanism may result in a weakened aortic wall with lacking ability to react on reinstating pulsatile blood flow.
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页数:16
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