GRWD1 promotes cell proliferation and migration in non-small cell lung cancer by activating the Notch pathway

被引：16

作者：

Wang, Qiongzi ^{[1
,2
]}

Ren, Hongjiu ^{[1
,2
]}

Xu, Yitong ^{[1
,2
]}

Jiang, Jun ^{[1
,2
]}

Wudu, Muli ^{[1
,2
]}

Liu, Zongang ^{[3
]}

Su, Hongbo ^{[1
,2
]}

Jiang, Xizi ^{[1
,2
]}

Zhang, Yao ^{[1
,2
]}

Zhang, Bo ^{[1
,2
]}

Qiu, Xueshan ^{[1
,2
]}

机构：

[1] China Med Univ, Affiliated Hosp 1, Dept Pathol, Shenyang, Peoples R China

[2] China Med Univ, Coll Basic Med Sci, Shenyang, Peoples R China

[3] China Med Univ, Shengjing Hosp, Dept Thorac Surg, 36 Sanhao St, Shenyang, Peoples R China

来源：

EXPERIMENTAL CELL RESEARCH | 2020年 / 387卷 / 02期

关键词：

Non-small cell king cancer; GRWD1; Cell proliferation; Cell migration; Notch pathway; PROTEIN GRWD1; WD40; PROTEINS; ADENOCARCINOMA; EXPRESSION; P53; IDENTIFICATION; INTEGRATION; INHIBITION; GTPASES; SNAIL;

D O I：

10.1016/j.yexcr.2019.111806

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

GRWD1 is a member of the WD repeat protein family that is over-expressed in various cancer cell lines and associated with poor prognosis in patients with cancer. However, its biological function and mechanism in non-small cell lung cancer (NSCLC) remain unclear. In this study, we aimed to elucidate the role of GRWD1 in NSCLC. Immunohistochemistry on tumor specimens from 170 patients showed that GRWD1 is highly expressed in NSCLC tissues and positively correlated with tumor size, lymph node metastasis, and P-TNM stage, but negatively correlated with differentiation and prognosis. We found that GRWD1 promotes cell colony formation by affecting the expression of Cyclin B1, CDK1, and p27 and inducing G2/M transition. GRWD1 was also found to stimulate cell migration through FthoA, RhoC, and CDC42, and induce epithelial mesenchymal transition by affecting the expression of E-cadherin, N-cadherin, Vimentin, Snail, Zeb1, and ZO-1. Our results indicated that the GRWD1 can activate the Notch signaling pathway by affecting the Notch intracellular domain and promoting the expression of Hesl. Our use of DAPT to suppress Notch signaling confirmed that GRWD1 promotes the progression of NSCLC through the Notch signaling pathway and may be a potential prognostic biomarker and therapeutic target for this disease.

引用

页数：13

共 50 条

[41] Reticulocalbin 1 is required for proliferation and migration of non-small cell lung cancer cells regulated by osteoblast-conditioned medium [J].

Fu, Haijing ;

Chen, Rui ;

Wang, Yue ;

Xu, Yang ;

Xia, Chun ;

Zhang, Bing .

JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2021, 25 (24) :11198-11211

[42] MicroRNA-4286 promotes cell proliferation, migration, and invasion via PTEN regulation of the PI3K/Akt pathway in non-small cell lung cancer [J].

Ling, Chunhua ;

Wang, Xueting ;

Zhu, Jianjie ;

Tang, Haicheng ;

Du, Wenwen ;

Zeng, Yuanyuan ;

Sun, Lin ;

Huang, Jian-An ;

Liu, Zeyi .

CANCER MEDICINE, 2019, 8 (07) :3520-3531

[43] ABCC4 is required for cell proliferation and tumorigenesis in non-small cell lung cancer [J].

Zhao, Xiaoting ;

Guo, Yinan ;

Yue, Wentao ;

Zhang, Lina ;

Gu, Meng ;

Wang, Yue .

ONCOTARGETS AND THERAPY, 2014, 7 :343-351

[44] PTP1B promotes cell proliferation and metastasis through activating src and ERK1/2 in non-small cell lung cancer [J].

Liu, Hongbing ;

Wu, Ying ;

Zhu, Suhua ;

Liang, Wenjun ;

Wang, Zhaofeng ;

Wang, Yunfen ;

Lv, Tangfeng ;

Yao, Yanwen ;

Yuan, Dongmei ;

Song, Yong .

CANCER LETTERS, 2015, 359 (02) :218-225

[45] Mesothelin promotes brain metastasis of non-small cell lung cancer by activating MET [J].

Xia, Shengkai ;

Duan, Wenzhe ;

Xu, Mingxin ;

Li, Mengqi ;

Tang, Mengyi ;

Wei, Song ;

Lin, Manqing ;

Li, Encheng ;

Liu, Wenwen ;

Wang, Qi .

JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH, 2024, 43 (01)

[46] Regulatory Effects of Siegesbeckia glabrescens on Non-Small Cell Lung Cancer Cell Proliferation and Invasion [J].

Lee, Ha Neul ;

Joo, Ji-Hye ;

Oh, Joa Sub ;

Choi, Shin Wook ;

Seo, Dong-Wan .

AMERICAN JOURNAL OF CHINESE MEDICINE, 2014, 42 (02) :453-463

[47] HOXC8 promotes proliferation and migration through transcriptional upregulation of TGFβ1 in non-small cell lung cancer [J].

Liu, Houli ;

Zhang, Mingsheng ;

Xu, Shanshan ;

Zhang, Jie ;

Zou, Jin ;

Yang, Chenchen ;

Zhang, Yang ;

Gong, Chen ;

Kai, Yuanzhong ;

Li, Yong .

ONCOGENESIS, 2018, 7

[48] MicroRNA-454 enhances proliferation and migration in non-small cell lung cancer cells [J].

Li, Yun ;

Chen, Quanmei ;

Zhang, Chunxue ;

Yang, Chanjuan ;

Bu, Youquan ;

Zhang, Yunmei .

INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE, 2019, 12 (06) :6932-6945

[49] Musashi1 Promotes Non-Small Cell Lung Carcinoma Malignancy and Chemoresistance via Activating the Akt Signaling Pathway [J].

Lang, Yaoguo ;

Kong, Xianglong ;

He, Changjun ;

Wang, Fengjiao ;

Liu, Benkun ;

Zhang, Shibo ;

Ning, Jinfeng ;

Zhu, Kaibin ;

Xu, Shidong .

CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 2017, 44 (02) :455-466

[50] LncRNA PKMYT1AR promotes cancer stem cell maintenance in non-small cell lung cancer via activating Wnt signaling pathway [J].

He, Yaomei ;

Jiang, Xiulin ;

Duan, Lincan ;

Xiong, Qiuxia ;

Yuan, Yixiao ;

Liu, Peishen ;

Jiang, Liping ;

Shen, Qiushuo ;

Zhao, Song ;

Yang, Cuiping ;

Chen, Yongbin .

MOLECULAR CANCER, 2021, 20 (01)

← 1 2 3 4 5 →