Genotypic and phenotypic characteristics of Streptococcus pneumoniae from community-acquired pneumonia patients and healthy asymptomatic participants in Sichuan province, China

被引：8

作者：

Peng, Shihui ^{[1
]}

Ren, Hongyu ^{[2
]}

Deng, Jianping ^{[3
]}

Zhao, Na ^{[2
]}

Li, Yinan ^{[2
]}

Li, Ming ^{[4
]}

Yuan, Qiwu ^{[4
]}

Zhang, Zhengdong ^{[3
]}

Luo, Longze ^{[5
]}

Zeng, Linzi ^{[5
]}

Wang, Bin ^{[3
]}

Zou, Nianli ^{[3
]}

Gu, Changguo ^{[6
]}

Huang, Xin ^{[6
]}

Liao, Zheng ^{[1
]}

Chen, Shenen ^{[1
]}

Chen, Haiying ^{[1
]}

Li, Qun ^{[3
]}

Qin, Tian ^{[2
]}

机构：

[1] Nanchang Ctr Dis Control & Prevent, Jiangxi Prov Key Lab Anim Origin & Vector Born Di, State Key Lab Infect Dis Prevent & Control, Collaborat Unit Field Epidemiol, Nanchang, Jiangxi, Peoples R China

[2] Chinese Ctr Dis Control & Prevent, Natl Inst Communicable Dis Control & Prevent, State Key Lab Infect Dis Prevent & Control, 155,Chang Bai Rd, Beijing 102206, Peoples R China

[3] Zigong Ctr Dis Control & Prevent, Zigong, Peoples R China

[4] Chengdu Ctr Dis Control & Prevent, Chengdu, Peoples R China

[5] Sichuan Ctr Dis Control & Prevent, Chengdu, Peoples R China

[6] Fifth Peoples Hosp Chengdu, Chengdu, Peoples R China

来源：

BMC INFECTIOUS DISEASES | 2021年 / 21卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Streptococcus pneumoniae; Serotype; Antimicrobial susceptibility; Virulence gene; Molecular epidemiology; PNEUMOCOCCAL CONJUGATE VACCINE; ANTIMICROBIAL RESISTANCE; HAEMOPHILUS-INFLUENZAE; ANTIBIOTIC-RESISTANCE; SEROTYPE DISTRIBUTION; CHILDREN; CARRIAGE; SUSCEPTIBILITY; SURVEILLANCE; VIRULENCE;

D O I：

10.1186/s12879-021-06737-w

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Background Streptococcus pneumoniae (S. pneumoniae) is the common cause of community-acquired pneumonia (CAP) and is also found in the upper respiratory tract of healthy people. Hence, the study aimed to compare the serotypes, virulence/pili genes, and antibiotic susceptibility of S. pneumoniae from healthy asymptomatic participants and CAP patients. Methods Streptococcus pneumoniae were retrospectively collected from health asymptomatic participants and CAP patients in Sichuan, China. The serotypes were tested by multiplex polymerase chain reaction (PCR) or Quellung reaction. Antibiotic susceptibility testing was performed using the broth microdilution method. The molecular epidemiology of S. pneumoniae was analyzed by multilocus sequence typing (MLST). Additionally, the presence of virulence/pili genes were detected using PCR. Results A total of 83 pneumococcal isolates were collected in the current study. Of these, 52 and 31 isolates were from healthy asymptomatic participants and CAP patients, respectively. Most of S. pneumoniae were resistant to erythromycin (ERY), clindamycin (CLI), tetracycline (TET) and trimethoprim-sulfamethoxazole (SXT). 90.4% isolates were classified as multidrug resistant (MDR). The predominant serotypes were 3, 19F and 19A in the CAP carriers, whereas 3, 6 and 19F were the main serotypes among the asymptomatic carriers. The overall coverage rates of pneumococcal conjugate vaccine (PCV) 10 and PCV13 serotypes were 34.9% and 66.3%, respectively. The predominant sequence types (STs) were ST271, ST320, and ST3397. There were significant differences in some resistance and virulence characteristics between CAP patients and asymptomatic carriers. Additionally, clonal complex (CC) 271 strains had higher percentage in resistance to cefuroxime (CXM) and cefotaxime (CEF), meropenem (MER) and cefepime (CFP), which mainly carried the rlrA and sipA genes. Conclusions High coverage rate of PCV13 and high prevalence of MDR indicated the necessity to expand immunization with PCV13 and rationally use the antibiotics in Sichuan, China. Importantly, long-term surveillance should be conducted to assess effectiveness brought by vaccines. Our findings may supply new guidance for developing new pneumococcal vaccines.

引用

页数：11

共 48 条

[41] Serotype distribution, antibiotic resistance pattern, and multilocus sequence types of invasive Streptococcus pneumoniae isolates in two tertiary pediatric hospitals in Beijing prior to PCV13 availability [J].