Stable DOPG/Glycyrrhizin Vesicles with a Wide Range of Mixing Ratios: Structure and Stability as Seen by Scattering Experiments and Cryo-TEM

被引：6

作者：

Dargel, Carina ^{[1
,3
]}

Graebitz-Braeuer, Friederike ^{[1
]}

Geisler, Ramsia ^{[1
,4
]}

Fandrich, Pascal ^{[1
]}

Hannappel, Yvonne ^{[1
]}

Porcar, Lionel ^{[2
]}

Hellweg, Thomas ^{[1
]}

机构：

[1] Bielefeld Univ, Phys & Biophys Chem, Univ Str 25, D-33615 Bielefeld, Germany

[2] Inst Laue Langevin, 71 Ave Martyrs CS 20156, F-38042 Grenoble 9, France

[3] Univ Munster, Inst Phys Chem, Corrensstr 28-30, D-48149 Munster, Germany

[4] Univ Hosp Frankfurt, Med Dept Hematol Oncol & Infect Dis 2, D-60590 Frankfurt, Germany

来源：

MOLECULES | 2021年 / 26卷 / 16期

关键词：

DOPG; glycyrrhizin; small unilamellar vesicle (SUV); SAXS and SANS; WAXS; SMALL-ANGLE NEUTRON; MEMBRANE-LIPIDS; GLYCYRRHIZIC ACID; SAFETY ASSESSMENT; PHASE-BEHAVIOR; SAPONINS; PHOSPHATIDYLGLYCEROL; PERMEABILITY; THICKNESS; BILAYERS;

D O I：

10.3390/molecules26164959

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Phosphatidylglycerols represent a large share of the lipids in the plasmamembrane of procaryotes. Therefore, this study investigates the role of charged lipids in the plasma membrane with respect to the interaction of the antiviral saponin glycyrrhizin with such membranes. Glycyrrhizin is a natural triterpenic-based surfactant found in licorice. Vesicles made of 1,2-dioleoyl-sn-glycero-3-phospho-rac-(1'-glycerol) (DOPG)/glycyrrhizin are characterized by small-angle scattering with neutrons and X-rays (SANS and SAXS). Small-angle scattering data are first evaluated by the model-independent modified Kratky-Porod method and afterwards fitted by a model describing the shape of small unilamellar vesicles (SUV) with an internal head-tail contrast. Complete miscibility of DOPG and glycyrrhizin was revealed even at a ratio of lipid:saponin of 1:1. Additional information about the chain-chain correlation distance of the lipid/saponin mixtures in the SUV structures is obtained from wide-angle X-ray scattering (WAXS).

引用

页数：17

共 73 条

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