Multiplexed and Reiterative Fluorescence Labeling via DNA Circuitry

被引：18

作者：

Duose, Dzifa Y. ^{[1
]}

Schweller, Ryan M. ^{[1
]}

Hittelman, Walter N. ^{[3
]}

Diehl, Michael R. ^{[1
,2
]}

机构：

[1] Rice Univ, Dept Bioengn, Houston, TX 77005 USA

[2] Rice Univ, Dept Chem, Houston, TX 77005 USA

[3] Univ Texas Houston, MD Anderson Canc Ctr, Dept Expt Therapeut, Houston, TX 77030 USA

来源：

BIOCONJUGATE CHEMISTRY | 2010年 / 21卷 / 12期

基金：

美国国家科学基金会;

关键词：

HYBRIDIZATION; CELLS;

D O I：

10.1021/bc100348q

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A class of reactive DNA circuits was adapted as erasable molecular imaging probes that allow fluorescent reporting complexes to be assembled and disassembled on a biological specimen. Circuit reactions are sequence-dependent and therefore facilitate multiplexed (multicolor) detection. Yet, the ability to disassemble reporting complexes also allows fluorophores to be removed and new circuit complexer to be used to label additional markers. Thus. these probes present opportunities to increase the total number of molecular targets that can be visualized on a biological sample by allowing multiple rounds of fluorescence microscopy to be performed.

引用

收藏

页码：2327 / 2331

页数：5

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