Analysis of the tomato mild mottle virus genome indicates that it is the most divergent member of the genus Ipomovirus (family Potyviridae)

被引：15

作者：

Abraham, Adane ^{[2
]}

Menzel, Wulf ^{[1
]}

Vetten, H. Josef ^{[3
]}

Winter, Stephan ^{[1
]}

机构：

[1] Deutsch Sammlung Mikroorganism Zellkultur GmbH, Leibniz Inst German Collect Microorganisms & Cell, Braunschweig, Germany

[2] Ethiopian Inst Agr Res, Holetta Agr Res Ctr, Addis Ababa, Ethiopia

[3] Julius Kuehn Inst Cultivated Plants, Braunschweig, ME, Germany

来源：

ARCHIVES OF VIROLOGY | 2012年 / 157卷 / 02期

关键词：

VEIN YELLOWING VIRUS; BROWN-STREAK-VIRUS; HC-PRO; TRANSMISSION; MUTATIONS; RNA; PROTEINASE; DISEASE; BINDING; ABSENCE;

D O I：

10.1007/s00705-011-1167-9

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The complete genome of a tomato mild mottle virus (ToMMV) isolate was analysed, and some biological features were characterized. The ssRNA genome of ToMMV from Ethiopia encompasses 9283 nucleotides (excluding the 3' poly(A) tail) and encodes a polyprotein of 3011 amino acids. Phylogenetic and pairwise comparisons with other members of the family Potyviridae revealed that ToMMV is the most divergent member of the genus Ipomovirus, with a genome organization similar to that of members of the species Sweet potato mild mottle virus, the type species of the genus. In contrast to earlier reports, ToMMV isolates from Yemen and Ethiopia were not transmitted by the aphid Myzus persicae, but they were transmitted very erratically by the whitefly Bemisia tabaci. A comparison of the 3'-proximal sequences of different isolates provided evidence for geographically associated genetic variation.

引用

页码：353 / 357

页数：5

共 25 条

[1] Chickpea chlorotic stunt virus: A new polerovirus infecting cool-season food legumes in Ethiopia [J].

Abraham, AD ;

Menzel, W ;

Lesemann, DE ;

Varrelmann, M ;

Vetten, HJ .

PHYTOPATHOLOGY, 2006, 96 (05) :437-446

[2] Overview and analysis of the polyprotein cleavage sites in the family Potyviridae [J].

Adams, MJ ;

Antoniw, JF ;

Beaudoin, F .

MOLECULAR PLANT PATHOLOGY, 2005, 6 (04) :471-487

[3] Molecular criteria for genus and species discrimination within the family Potyviridae [J].

Adams, MJ ;

Antoniw, JF ;

Fauquet, CM .

ARCHIVES OF VIROLOGY, 2005, 150 (03) :459-479

[4] MUTATIONAL ANALYSIS OF THE COAT PROTEIN N-TERMINAL AMINO-ACIDS INVOLVED IN POTYVIRUS TRANSMISSION BY APHIDS [J].

ATREYA, PL ;

LOPEZMOYA, JJ ;

CHU, MH ;

ATREYA, CD ;

PIRONE, TP .

JOURNAL OF GENERAL VIROLOGY, 1995, 76 :265-270

[5] Mutations in the potyvirus helper component protein: effects on interactions with virions and aphid stylets [J].

Blanc, S ;

Ammar, ED ;

Garcia-Lampasona, S ;

Dolja, VV ;

Llave, C ;

Baker, J ;

Pirone, TP .

JOURNAL OF GENERAL VIROLOGY, 1998, 79 :3119-3122

[6] A universal PCR primer to detect members of the Potyviridae and its use to examine the taxonomic status of several members of the family [J].

Chen, J ;

Adams, MJ .

ARCHIVES OF VIROLOGY, 2001, 146 (04) :757-766

[7] An overlapping essential gene in the Potyviridae [J].

Chung, Betty Y. -W. ;

Miller, W. Allen ;

Atkins, John F. ;

Firth, Andrew E. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (15) :5897-5902

[8]

Dombrowsky A, 2011, P 4 C INT WORK GROUP

[9] Helper component-transcomplementation in the vector transmission of plant viruses [J].

Froissart, R ;

Michalakis, Y ;

Blanc, S .

PHYTOPATHOLOGY, 2002, 92 (06) :576-579

[10] A RANDOM-PCR METHOD (RPCR) TO CONSTRUCT WHOLE CDNA LIBRARY FROM LOW AMOUNTS OF RNA [J].

FROUSSARD, P .

NUCLEIC ACIDS RESEARCH, 1992, 20 (11) :2900-2900

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