Effects of EGCG on proliferation and apoptosis of gastric cancer SGC7901 cells via down-regulation of HIF-1α and VEGF under a hypoxic state

被引：9

作者：

Fu, J-D ^{[1
]}

Yao, J-J ^{[1
]}

Wang, H. ^{[2
]}

Cui, W-G ^{[1
]}

Leng, J. ^{[1
]}

Ding, L-Y ^{[1
]}

Fan, K-Y ^{[2
]}

机构：

[1] Jining Med Univ, Peoples Hosp Rizhao, Affiliated Clin Hosp, Dept Digest Dis, Rizhao, Shandong, Peoples R China

[2] Jining Med Coll, Dept Infect Dis, Rizhao Peoples Hosp, Rizhao, Shandong, Peoples R China

来源：

EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES | 2019年 / 23卷 / 01期

关键词：

EGCG; Hypoxia; Gastric cancer cells; Hypoxia-inducible factor-1 alpha; Vascular endothelial growth factor; ENDOTHELIAL GROWTH-FACTOR; GREEN TEA; TUMOR ANGIOGENESIS; GENE-EXPRESSION; ACTIVATION; PROTEIN; INHIBITION; (-)-EPIGALLOCATECHIN-3-GALLATE; STABILIZATION; SUPPRESSION;

D O I：

暂无

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

OBJECTIVE: To investigate the effects of epigallocatechin-3-gallate (EGCG) on proliferation and apoptosis of human gastric cancer SGC7901 cells under a hypoxic state. MATERIALS AND METHODS: Human gastric cancer SGC7901 cells were sub-cultured, and the cobalt chloride (CoCl2) hypoxia model was established. The blank control group (normoxia group), hypoxia control group (hypoxia group) and hypoxia + different concentrations of EGCG subgroups (20, 40, 60, 80, 100 mu g/mL EGCG) were set up. Cell viability was detected via methyl thiazolyl tetrazolium (MTT) assay, apoptosis was detected via flow cytometry, and expressions of hypoxia-inducible factor-1 alpha (HIF-1 alpha) and vascular endothelial growth factor (VEGF) were detected via reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting. RESULTS: Relatively low concentrations of EGCG (20-80 mu g/mL) presented no significant inhibiting effect on SGC7901 cell growth within a short time (24 h) (p>0.05). The increasing concentration of EGCG inhibited cell proliferation under a hypoxia state (p<0.05). EGCG induced apoptosis in a dose-dependent manner under hypoxia (p<0.05). EGCG could significantly impede expressions of HIF-1 alpha and VEGF proteins (p<0.05), and downregulate the level of VEGF mRNA (p<0.05), but it showed no significant effect on the HIF-1 alpha mRNA expression (p>0.05). CONCLUSIONS: EGCG inhibited cell proliferation under hypoxia via the downregulation of HIF-1 alpha and its downstream target gene VEGF levels, providing a theoretical basis for the early diagnosis and treatment of gastric cancer in clinic.

引用

页码：155 / 161

页数：7