Fluorescence line-narrowing spectroscopy for probing purposes in bioanalytical and environmental chemistry

被引：16

作者：

Ariese, Freek ^{[1
]}

Bader, Arjen N. ^{[1
]}

Gooijer, Cees ^{[1
]}

机构：

[1] Vrije Univ Amsterdam, Ctr Laser, Dept Analyt Chem & Appl Spect, NL-1081 HV Amsterdam, Netherlands

来源：

TRAC-TRENDS IN ANALYTICAL CHEMISTRY | 2008年 / 27卷 / 02期

关键词：

benzo[a]pyrene; cryogenic technique; estrogen receptor; high-resolution fluorescence; humic substance; monoclonal antibody; PAH-DNA adduct; PAH metabolite; polyaromatic hydrocarbon; porphyrin;

D O I：

10.1016/j.trac.2008.01.002

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Fluorescence line-narrowing spectroscopy (FLNS) is a cryogenic technique that can be used to obtain high-resolution fluorescence spectra. The technique has mainly been used for fingerprint identification. However, this review focuses on the trend to use FLNS for probing purposes: obtaining information on interactions with the local environment or determining conformations. We briefly discuss the basics of FLNS, solute-matrix interactions and important instrumental aspects. We describe examples from various bioanalytical fields: DNA adducts of the aromatic carcinogen benzo[a]pyrene (BP); binding of BP metabolites to monoclonal antibodies or the estrogen receptor; FLNS studies of porphyrin-containing proteins; and, photosynthetic systems. As an illustration from the field of environmental chemistry, we discuss the use of luminescence line-narrowing spectroscopy - a special mode of FLNS - to study the complexation of lanthanides to humic substances. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：127 / 138

页数：12

共 54 条

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