Structural mechanisms of centromeric nucleosome recognition by the kinetochore protein CENP-N

被引：88

作者：

Chittori, Sagar ^{[1
]}

Hong, Jingjun ^{[2
]}

Saunders, Hayden ^{[2
]}

Feng, Hanqiao ^{[2
]}

Ghirlando, Rodolfo ^{[3
]}

Kelly, Alexander E. ^{[2
]}

Bai, Yawen ^{[2
]}

Subramaniam, Sriram ^{[1
]}

机构：

[1] NCI, Lab Cell Biol, CCR, NIH, Bethesda, MD 20892 USA

[2] NCI, Lab Biochem & Mol Biol, CCR, NIH, Bethesda, MD 20892 USA

[3] NIDDK, Lab Mol Biol, NIH, Bethesda, MD 20892 USA

来源：

SCIENCE | 2018年 / 359卷 / 6373期

关键词：

A NUCLEOSOMES; CHROMATIN; HISTONE; ORGANIZATION; ARCHITECTURE; HETERODIMER; COMPLEX;

D O I：

10.1126/science.aar2781

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Accurate chromosome segregation requires the proper assembly of kinetochore proteins. A key step in this process is the recognition of the histone H3 variant CENP-A in the centromeric nucleosome by the kinetochore protein CENP-N. We report cryo-electron microscopy (cryo-EM), biophysical, biochemical, and cell biological studies of the interaction between the CENP-A nucleosome and CENP-N. We show that human CENP-N confers binding specificity through interactions with the L1 loop of CENP-A, stabilized by electrostatic interactions with the nucleosomal DNA. Mutational analyses demonstrate analogous interactions in Xenopus, which are further supported by residue-swapping experiments involving the L1 loop of CENP-A. Our results are consistent with the coevolution of CENP-N and CENP-A and establish the structural basis for recognition of the CENP-A nucleosome to enable kinetochore assembly and centromeric chromatin organization.

引用

页码：339 / +

页数：5

共 30 条

[21] A super-resolution map of the vertebrate kinetochore [J].

Ribeiro, Susana Abreu ;

Vagnarelli, Paola ;

Dong, Yimin ;

Hori, Tetsuya ;

McEwen, Bruce F. ;

Fukagawa, Tatsuo ;

Flors, Cristina ;

Earnshaw, William C. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (23) :10484-10489

[22] Solution structure of the tumor necrosis factor receptor-1 death domain [J].

Sukits, SF ;

Lin, LL ;

Hsu, S ;

Malakian, K ;

Powers, R ;

Xu, GY .

JOURNAL OF MOLECULAR BIOLOGY, 2001, 310 (04) :895-906

[23] The Architecture of CCAN Proteins Creates a Structural Integrity to Resist Spindle Forces and Achieve Proper Intrakinetochore Stretch [J].

Suzuki, Aussie ;

Badger, Benjamin L. ;

Wan, Xiaohu ;

DeLuca, Jennifer G. ;

Salmon, Edward D. .

DEVELOPMENTAL CELL, 2014, 30 (06) :717-730

[24] Crystal structure of the human centromeric nucleosome containing CENP-A [J].

Tachiwana, Hiroaki ;

Kagawa, Wataru ;

Shiga, Tatsuya ;

Osakabe, Akihisa ;

Miya, Yuta ;

Saito, Kengo ;

Hayashi-Takanaka, Yoko ;

Oda, Takashi ;

Sato, Mamoru ;

Park, Sam-Yong ;

Kimura, Hiroshi ;

Kurumizaka, Hitoshi .

NATURE, 2011, 476 (7359) :232-U135

[25] Stepwise unfolding supports a subunit model for vertebrate kinetochores [J].

Vargiu, Giulia ;

Makarov, Alexandr A. ;

Allan, James ;

Fukagawa, Tatsuo ;

Booth, Daniel G. ;

Earnshaw, William C. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2017, 114 (12) :3133-3138

[26] Crystal Structures of Nucleosome Core Particles Containing the '601' Strong Positioning Sequence [J].

Vasudevan, Dileep ;

Chua, Eugene Y. D. ;

Davey, Curt A. .

JOURNAL OF MOLECULAR BIOLOGY, 2010, 403 (01) :1-10

[27] Centromeres: unique chromatin structures that drive chromosome segregation [J].

Verdaasdonk, Jolien S. ;

Bloom, Kerry .

NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2011, 12 (05) :320-332

[28] Insights from biochemical reconstitution into the architecture of human kinetochores [J].

Weir, John R. ;

Faesen, Alex C. ;

Klare, Kerstin ;

Petrovic, Arsen ;

Basilico, Federica ;

Fischboeck, Josef ;

Pentakota, Satyakrishna ;

Keller, Jenny ;

Pesenti, Marion E. ;

Pan, Dongqing ;

Vogt, Doro ;

Wohlgemuth, Sabine ;

Herzog, Franz ;

Musacchio, Andrea .

NATURE, 2016, 537 (7619) :249-+

[29] Molecular basis for the interaction between Integrator subunits IntS9 and IntS11 and its functional importance [J].

Wu, Yixuan ;

Albrecht, Todd R. ;

Baillat, David ;

Wagner, Eric J. ;

Tong, Liang .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2017, 114 (17) :4394-4399

[30] Structural basis for recognition of centromere histone variant CenH3 by the chaperone Scm3 [J].

Zhou, Zheng ;

Feng, Hanqiao ;

Zhou, Bing-Rui ;

Ghirlando, Rodolfo ;

Hu, Kaifeng ;

Zwolak, Adam ;

Jenkins, Lisa M. Miller ;

Xiao, Hua ;

Tjandra, Nico ;

Wu, Carl ;

Bai, Yawen .

NATURE, 2011, 472 (7342) :234-237

← 1 2 3 →