The Protective Effect of Adenosine-Preconditioning on Paraquat-Induced Damage inCaenorhabditis elegans

Adenosine plays an important role in the physiological and pathological conditions of the body by combining different types of adenosine receptors widely distributed in various tissues in the body. In present study, an acute model for paraquat-poisoning inCaenorhabditis eleganswas established for quantitative assessment via a time-dose-mortality (TDM) modeling technique with various paraquat doses over 8 hours. Adenosine was first used to precondition at high, medium, and low concentrations and the survival rate ofC. eleganswas recorded to evaluate adenosine antistress protection against paraquat damage. The results revealed that the TDM model was good for the quantitative assessment of paraquat-poisoning onC. elegansbased on the Hosmer-Lemeshow test for homogeneity of modeling (P= .38). The survival rates of adenosine-preconditionedC. eleganshave a dose-dependent association with adenosine concentration. At 3000 mu M (high concentration) and 300 mu M (medium concentration), adenosine-preconditionedC. elegansstill had survival rates of 5.38% +/- 1.68% and 5.0% +/- 1.19% in the subsequent 8 hours observation period. On the contrary, the survival rates of those receiving 30 mu M (low concentration) and the 0 mu M (unpreconditioned treatment) were zero. To conclude, adenosine preconditioning had protective effects onC. elegansintoxicated with paraquat by decreasing its mortality rate.