Effect of distillery effluent on seed germination and seedling growth of Vigna mungo (L.) hepper

In the laboratory culture experiment, the effect of different concentrations of distillery effluent on seed germination and seedling growth (0,5,10,25,50, 75 and 100ml/l of effluent) of Vigna mungo (variety T-9 and ADT-1) was studied on 71 day. The seeds were surface sterilized using 0.1% mercuric chloride (HgCl2) for two minutes. The inner surface of sterilized Petri plates lined with filter paper. Twenty five seeds were sown in arranged at equal distance. Control plants were maintained separately. Equal volume of various concentrations (5-100 ml/l) of distillery effluent were poured into respective Petri plates. Each treatment including the control was replicated five times. The Petri plates were incubated in seed germinator at (28 +/- 20 degrees C). Distillery effluent at all levels (5-10 ml/l) tested, the seed germination and seedling growth parameters such as root length, lateral root and shoot length, vigour index and tolerance index were higher 5 and 10 ml/l of treatment then that of control and other (25, 50, 75 and 100 ml/l) concentrations of treated plants. Phytotoxicity percentage of the black gram plants increased with an increase of distillery effluents.