Towards an alternative testing strategy for nanomaterials used in nanomedicine: Lessons from NanoTEST

被引：56

作者：

Dusinska, M. ^{[1
]}

Boland, S. ^{[2
]}

Saunders, M. ^{[3
]}

Juillerat-Jeanneret, L. ^{[4
]}

Tran, L. ^{[5
]}

Pojana, G. ^{[6
,7
]}

Marcomini, A. ^{[7
]}

Volkovova, K. ^{[8
]}

Tulinska, J. ^{[8
]}

Knudsen, L. E. ^{[9
]}

Gombau, L. ^{[10
]}

Whelan, M. ^{[11
]}

Collins, A. R. ^{[12
]}

Marano, F. ^{[2
]}

Housiadas, C. ^{[13
]}

Bilanicova, D. ^{[6
,7
]}

Kenzaoui, B. Halamoda ^{[4
,11
]}

Carreira, S. Correia ^{[14
]}

Magdolenova, Z. ^{[1
]}

Fjellsbo, L. M. ^{[1
]}

Huk, A. ^{[1
]}

Handy, R. ^{[15
]}

Walker, L. ^{[16
]}

Barancokova, M. ^{[8
]}

Bartonova, A. ^{[1
]}

Burello, E. ^{[11
,17
]}

Castell, J. ^{[10
]}

Cowie, H. ^{[5
]}

Drlickova, M. ^{[8
,18
]}

Guadagnini, R. ^{[2
]}

Harris, G. ^{[11
]}

Harju, M. ^{[1
]}

Heimstad, E. S. ^{[1
]}

Hurbankova, M. ^{[8
]}

Kazimirova, A. ^{[8
]}

Kovacikova, Z. ^{[8
]}

Kuricova, M. ^{[8
]}

Liskova, A. ^{[8
]}

Milcamps, A. ^{[11
]}

Neubauerova, E. ^{[8
]}

Palosaari, T. ^{[11
]}

Papazafiri, P. ^{[19
]}

Pilou, M. ^{[14
]}

Poulsen, M. S. ^{[9
]}

Ross, B. ^{[5
]}

Runden-Pran, E. ^{[1
]}

Sebekova, K. ^{[20
]}

Staruchova, M. ^{[8
]}

Vallotto, D. ^{[6
,7
]}

Worth, A. ^{[11
]}

机构：

[1] Norwegian Inst Air Res NILU, Hlth Effects Lab MILK, N-2007 Kjeller, Norway

[2] Univ Paris Diderot, Unit Funct & Adapt Biol BFA, Lab Mol & Cellular Responses Xenobiot RMCX, Sorbonne Paris Cite,UMR CNRS 8251, Paris, France

[3] Univ Hosp Bristol NHS Fdn Trust, St Michaels Hosp, Bioengn Innovat & Res Hub, Dept Med Phys & Bioengn,BIRCH, Bristol, Avon, England

[4] Univ Inst Pathol, Lausanne, Switzerland

[5] Inst Occupat Med, Edinburgh EH8 9SV, Midlothian, Scotland

[6] Univ Ca Foscari Venice, DFBC Dept Philosophy & Cultural Heritage, Venice, Italy

[7] Univ Ca Foscari Venice, DAIS Dept Environm Sci Informat & Stat, Venice, Italy

[8] Slovak Med Univ, Fac Med, Bratislava, Slovakia

[9] Univ Copenhagen, Inst Publ Hlth, Fac Hlth & Med Sci, Copenhagen, Denmark

[10] Leitat Technol Ctr, Barcelona, Spain

[11] Commiss European Communities, Joint Res Ctr, Inst Hlth & Consumer Protect, I-21020 Ispra, VA, Italy

[12] Univ Oslo, Dept Nutr, Oslo, Norway

[13] NCSR Demokritos, Thermal Hydraul & Multiphase Flows Lab, Inst Nucl & Radiol Sci & Technol Energy & Safety, Aghia Paraskevi, Greece

[14] Univ Bristol, Bristol Ctr Funct Nanomat, Bristol, Avon, England

[15] Univ Plymouth, Sch Biomed & Biol Sci, Plymouth PL4 8AA, Devon, England

[16] Univ Bristol, Bristol Heart Inst, Sch Clin Sci, Bristol, Avon, England

[17] TNO, Computat Chem Grp, RAPID Dept Risk Anal Prod Dev, NL-3700 AJ Zeist, Netherlands

[18] Ctr Chem Subst & Preparat, Bratislava, Slovakia

[19] Univ Athens, Dept Biol, GR-15701 Athens, Greece

[20] Comenius Univ, Fac Med, Inst Mol Biomed, Bratislava, Slovakia

来源：

NANOTOXICOLOGY | 2015年 / 9卷

关键词：

Hazard assessment; in vitro; nanoparticles; NanoTEST; testing strategy; IRON-OXIDE NANOPARTICLES; TITANIUM-DIOXIDE NANOPARTICLES; PLGA-PEO NANOPARTICLES; HUMAN COLON CELLS; IN-VITRO; ENGINEERED NANOPARTICLES; EPITHELIAL-CELLS; ENDOTHELIAL-CELLS; STRESS REACTION; COMET ASSAY;

D O I：

10.3109/17435390.2014.991431

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

In spite of recent advances in describing the health outcomes of exposure to nanoparticles (NPs), it still remains unclear how exactly NPs interact with their cellular targets. Size, surface, mass, geometry, and composition may all play a beneficial role as well as causing toxicity. Concerns of scientists, politicians and the public about potential health hazards associated with NPs need to be answered. With the variety of exposure routes available, there is potential for NPs to reach every organ in the body but we know little about the impact this might have. The main objective of the FP7 NanoTEST project (www.nanotest-fp7.eu) was a better understanding of mechanisms of interactions of NPs employed in nanomedicine with cells, tissues and organs and to address critical issues relating to toxicity testing especially with respect to alternatives to tests on animals. Here we describe an approach towards alternative testing strategies for hazard and risk assessment of nanomaterials, highlighting the adaptation of standard methods demanded by the special physicochemical features of nanomaterials and bioavailability studies. The work has assessed a broad range of toxicity tests, cell models and NP types and concentrations taking into account the inherent impact of NP properties and the effects of changes in experimental conditions using well-characterized NPs. The results of the studies have been used to generate recommendations for a suitable and robust testing strategy which can be applied to new medical NPs as they are developed.

引用

页码：118 / 132

页数：15

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