UV-A-induced AP-1 activation requires the Raf/ERK pathway in human NCTC 2544 keratinocytes

被引：26
作者：
Djavaheri-Mergny, M
Dubertret, L
机构：
[1] Inst Curie, INSERM U365, Rech Sect Biol, F-75231 Paris 05, France
[2] Hop St Louis, INSERM 523, Inst Rech Peau, F-75475 Paris, France
来源：
EXPERIMENTAL DERMATOLOGY | 2001年 / 10卷 / 03期
关键词：
UV-A; oxidative stress; ERK; Raf kinase; AP-1; activation; singlet oxygen;
D O I：
10.1034/j.1600-0625.2001.010003204.x
中图分类号：
R75 [皮肤病学与性病学];
学科分类号：
100206 ;
摘要：
UV-A irradiation causes a dose-dependent activation of ERK in human NCTC 2544 keratinocytes. The specific inhibition of either ERK activity or Raf kinase activity impedes the activation of AP-1 DNA binding induced by UV-A. In addition, UV-A raises AP-1 promoter transcriptional activity, which is downregulated in NCTC 2544 cells expressing an inactive mutant of Raf-1. We found that singlet oxygen might be one of the mediators in both UV-A-induced AP-1 DNA binding and transcriptional activity. These results strongly suggest that UV-A-induced AP-1 activity requires the Raf-ERK pathway and imply a singlet oxygen effector.
引用
收藏
页码：204 / 210
页数：7
相关论文
共 30 条
[1]  
[Anonymous], BIOCH BIOPHYS ACTA
[2]  
BASUMODAK S, 1993, CANCER RES, V53, P4505
[3]   SERUM-INDUCED, TPA-INDUCED, AND RAS-INDUCED EXPRESSION FROM AP-1/ETS-DRIVEN PROMOTERS REQUIRES RAF-1 KINASE [J].
BRUDER, JT ;
HEIDECKER, G ;
RAPP, UR .
GENES & DEVELOPMENT, 1992, 6 (04) :545-556
[4]   Identification of MAP kinase domains by redirecting stress signals into growth factor responses [J].
Brunet, A ;
Pouyssegur, J .
SCIENCE, 1996, 272 (5268) :1652-1655
[5]   UV-A-induced decrease in nuclear factor-κB activity in human keratinocytes [J].
Djavaheri-Mergny, M ;
Gras, MP ;
Mergny, JL ;
Dubertret, L .
BIOCHEMICAL JOURNAL, 1999, 338 :607-613
[6]   ULTRAVIOLET A DECREASES EPIDERMAL GROWTH-FACTOR (EGF) PROCESSING IN CULTURED HUMAN FIBROBLASTS AND KERATINOCYTES - INHIBITION OF EGF-INDUCED DIACYLGLYCEROL FORMATION [J].
DJAVAHERIMERGNY, M ;
MAZIERE, C ;
SANTUS, R ;
DUBERTRET, L ;
MAZIERE, JC .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1994, 102 (02) :192-196
[7]   Ultraviolet-A induces activation of AP-1 in cultured human keratinocytes [J].
DjavaheriMergny, M ;
Mergny, JL ;
Bertrand, F ;
Santus, R ;
Maziere, C ;
Dubertret, L ;
Maziere, JC .
FEBS LETTERS, 1996, 384 (01) :92-96
[8]  
Fisher G J, 1998, J Investig Dermatol Symp Proc, V3, P61
[9]   ULTRAVIOLET SUNLIGHT REACHING THE EARTHS SURFACE - A REVIEW OF RECENT RESEARCH [J].
FREDERICK, JE .
PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1993, 57 (01) :175-178
[10]   PYRIMIDINE DIMER FORMATION IN HUMAN-SKIN [J].
FREEMAN, SE ;
GANGE, RW ;
SUTHERLAND, JC ;
SUTHERLAND, BM .
PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1987, 46 (02) :207-212
123