Proteomics-driven identification of putative AfsR2-target proteins stimulating antibiotic biosynthesis in Streptomyces lividans

被引：14

作者：

Kim, CY

Park, HJ

Kim, ES ^{[1
]}

机构：

[1] Inha Univ, Dept Biol Engn, Inchon 402751, South Korea

[2] SongWon Envichem Inc, Seoul 135010, South Korea

来源：

BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | 2005年 / 10卷 / 03期

基金：

新加坡国家研究基金会;

关键词：

AfsR2; proteomics; antibiotic regulation; Streptomyces;

D O I：

10.1007/BF02932021

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

AfsR2, originally identified from 5treptomyces lividans, is a global regulatory protein which stimulates antibiotic biosynthesis. Through its stable chromosomal integration, the high level of gene expression of afsR2 significantly induced antibiotic production as well as the sporulation of S. lividans, implying the presence of yet-uncharacterized AfsR2-target proteins. To identify and evaluate the putative AfsR2-target proteins involved in antibiotic regulation, the proteomics-driven approach was applied to the wild-type S. lividans and the afsR2-integ rated actinorhodin overproducing strain. The 2D gel-electrophoresis gave approximately 340 protein spots showing different protein expression patterns between these two S. lividans strains. Further MALDI-TOF analysis revealed several AfsR2-target proteins, including glyceraldehyde-3-phosphate dehydrogenase, putative phosphate transport system regulator, guanosine pentaphosphate synthetase/polyribonucleotide nucleotidyltransferase, and superoxide dismutase, which suggests that the AfsR2 should be a pleiotropic regulatory protein which controls differential expressions of various kinds of genes in Streptomyces species.

引用

页码：248 / 253

页数：6

共 31 条

[1]

Cashel M., 1996, ESCHERICHIA COLI SAL, V1, P1458

[2] The ppGpp synthetase gene (relA) of Streptomyces coelicolor A3(2) play's a conditional role in antibiotic production and morphological differentiation [J].