Indonesian Honey Protein Isolation Apis dorsata dorsata and Tetragonula sp. as Antibacterial and Antioxidant Agent

Honey is a natural product that has many properties and been widely used for many theurapeutic purposes. Research on honey has been very rapid but not yet for Indonesia. Like local Indonesian honey Apis dorsata dorsata and Tetragonula sp. which has been widely consumed by the public but not yet known for certain efficacy o f each content. The function o f honey as antibacterial and antioxidant has not been specifically explained by the components contained in honey. Protein is one of the content o f honey that turned out to have activity as an antibacterial and antioxidant in certain types o f honey because o f it antimicrobial peptide. Testing o f honey activity as antibacterial and antioxidant through several stages including isolation. SDS-PAGE analysis, Bradford test antibacterial activity test with well diffusion method and antioxidant activity test by DPPH method. Bacteria used were gram-positive bacteria Staphylococcus aureus and gram negative Escherichia coli. After some experiment finally got protein isolation method that is in the form o f further concentration using Millipore membrane for honey Tetragonula sp. and membrane filtration dot blot for honey Apis dorsata dorsata. The Bradford assay showed that Apis dorsata dorsata honey contains protein <5 mu g / ml, while honey Tetragonula sp. has a protein content o f 97 mu g / ml. The characteristic profile of molecular weight o f the protein showed honey Tetragonula sp. has 3 protein bands composed o f 52, 96 - 61,9 kDa, 63.35 - 65,92 kDa and 86,16 - 91,4 kDa, whereas Apis dorsata dorsata honey has 5 protein bands consisting o f 45,2 - 46.6 kDa, 50,2 - 50,9 kDa. 62,5 - 62,9 kDa, 73,1 - 73,9 kDa. 83,9 - 86,9 kDa. Isolate honey protein Apis dorsata dorsata has no antioxidant and antibacterial activity (Staphylococcus aureus and Escherichia coli), whereas honey protein isolates Tetragomila sp. has antibacterial activity against Escherichia coli.