Cloning and Identification of Differentially Expressed Genes Associated with Smut in Sugarcane

被引：2

作者：

Song, Xiu-Peng ^{[1
]}

Tian, Dan-Dan ^{[3
]}

Chen, Ming-Hui ^{[2
]}

Qin, Zhen-Qiang ^{[1
]}

Wei, Jin-Ju ^{[1
]}

Wei, Chun-Yan ^{[1
]}

Zhang, Xiao-Qiu ^{[1
]}

Li, De-Wei ^{[1
]}

Yang, Li-Tao ^{[2
]}

Li, Yang-Rui ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Guangxi Acad Agr Sci,Sugarcane Res Ctr, Key Lab Sugarcane Biotechnol & Genet Improvement, Guangxi Key Lab Sugarcane Genet Improvement,Minis, Nanning 530007, Peoples R China

[2] Guangxi Univ, Coll Agr, State Key Lab Conservat & Utilizat Subtrop Agrobi, Nanning, Peoples R China

[3] Guangxi Acad Agr Sci, Biotechnol Res Inst, Nanning, Peoples R China

来源：

SUGAR TECH | 2018年 / 20卷 / 06期

关键词：

Sugarcane; Suppression subtractive hybridization; Smut; Subtractive library; Stress; TRANSCRIPTION FACTORS; CYTOCHROME-P450; GENE; PROTEIN-KINASE; STRESS; CHITINASE; RESPONSES; TISSUE;

D O I：

10.1007/s12355-018-0610-6

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The aim of this experiment was to evaluate the molecular mechanism of sugarcane response to the smut pathogen at the beginning of the pathogen infection of sugarcane seedlings, to explore related genes, and to provide useful information for developing rational strategies to control smut at early stages of disease development. A suppression subtractive hybridization library was constructed using cDNA synthesized from RNA extracted from normal stalks as driver and inoculated stalks as tester. The positive clones of the libraries were sequenced randomly, analyzed by BLAST, and classified by GO. A total of 248 positive clones were selected for sequencing, and a total of 224 EST sequences were obtained. In total, 188 ESTs were found to share a considerable homology with known genes, while the remaining 36 ESTs had no homology with known genes. In the Gene Ontology database, the unigenes were assigned functional descriptions; 152, 129, and 139 ESTs were, respectively, involved in cell component, molecular function, and biological process. Some genes related to a smut pathogen infection were obtained, while the SSH library was constructed. These genes reflected the regulation of sugarcane to smut pathogen and can be used as candidate genes.

引用

页码：717 / 724

页数：8

共 28 条

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