Trapping of proteins under physiological conditions in a nanopipette

被引：94
|
作者：
Clarke, RW [1 ]
White, SS [1 ]
Zhou, DJ [1 ]
Ying, LM [1 ]
Klenerman, D [1 ]
机构：
[1] Univ Cambridge, Dept Chem, Cambridge CB2 1TN, England
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2005年 / 44卷 / 24期
基金：
英国生物技术与生命科学研究理事会;
关键词：
biomolecule trapping; DNA; electrophoresis; nanotechnology; proteins;
D O I：
10.1002/anie.200500196
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
(Figure Presented) Caught in a trap and on camera: A nanopipette is used for electrodeless dielectrophoresis and clear evidence is shown, by using wide-field fluorescence imaging (see image), for the reversible trapping of Alexa-488-labeled proteins (protein G and immunoglobulin G) and also of the fluorophore alone. The dielectrophoretic concentration is enhanced by at least a factor of 300 for these fluorophore-labeled proteins. © 2005 Wiley-VCH Verlag GmbH & Co. KGaA.
引用
收藏
页码：3747 / 3750
页数：4
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