The PCR-based approaches for the detection of Marek's disease virus sequences in chickens

被引：0

作者：

Bossak, N ^{[1
]}

Karnuah, A ^{[1
]}

Furakawa, T ^{[1
]}

Yamamoto, Y ^{[1
]}

Mitsuhashi, T ^{[1
]}

机构：

[1] Natl Inst Anim Ind, Ibaraki 3050901, Japan

来源：

CURRENT PROGRESS ON MAREK'S DISEASE RESEARCH | 2001年

关键词：

chicken; Marek's disease virus; polymerase chain reaction;

D O I：

暂无

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

A sensitive complex method was employed for the screening of different populations of chickens for Marek's disease virus (MDV) sequences. The assay based on PCR analysis with primers for the ICP4, glycoprotein B (gB), thymidine kinase (TK) genes and for the MDV inverted repeats, was established using chickens inoculated with MDV. An unequal distribution of viral sequences among different organs and tissues of inoculated chicken was detected. MDV-affected birds showed varying degrees of degeneration of the investigated tissues. Using semi-quantitative PCR, a positive correlation between high level of degeneration of tissues and increased viral copy number was determined. MDV-gB sequences were also identified in 55% of investigated anonymous chicken (non-inoculated, representatives of different populations). This finding allowed us to conclude that the sensitive PCR-based approach can be used for the screening of chickens for MDV sequences but may have some limitations for disease diagnosis.

引用

页码：43 / 49

页数：7

共 23 条

[1]

Aminev AG, 1998, MOL BIOL+, V32, P774

[2] COMPLETE NUCLEOTIDE-SEQUENCE OF THE MAREKS-DISEASE VIRUS-ICP4 GENE [J].