Ethylene-Producing Bacteria That Ripen Fruit

被引：23

作者：

Digiacomo, Fabio ^{[1
]}

Girelli, Gabriele ^{[1
]}

Aor, Bruno ^{[1
]}

Marchioretti, Caterina ^{[1
]}

Pedrotti, Michele ^{[1
]}

Perli, Thomas ^{[1
]}

Tonon, Emil ^{[1
]}

Valentini, Viola ^{[1
]}

Avi, Damiano ^{[2
]}

Ferrentino, Giovanna ^{[3
]}

Dorigato, Andrea ^{[3
]}

Torre, Paola ^{[1
]}

Jousson, Olivier ^{[1
]}

Mansy, Sheref S. ^{[1
]}

Del Bianco, Cristina ^{[1
]}

机构：

[1] Univ Trento, Ctr Integrat Biol, I-38123 Trento, Italy

[2] Univ Trento, Dept Phys, Atom & Mol Phys Lab, I-38123 Trento, Italy

[3] Univ Trento, Dept Mat Engn & Ind Technol, I-38123 Trento, Italy

来源：

ACS SYNTHETIC BIOLOGY | 2014年 / 3卷 / 12期

关键词：

ethylene; fruit ripening; bioengineering; YF1; blue photoreceptor; iGEM;

D O I：

10.1021/sb5000077

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Ethylene is a plant hormone widely used to ripen fruit. However, the synthesis, handling, and storage of ethylene are environmentally harmful and dangerous. We engineered E. coli to produce ethylene through the activity of the ethylene-forming enzyme (EFE) from Pseudomonas syringae. EFE converts a citric acid cycle intermediate, 2-oxoglutarate, to ethylene in a single step. The production of ethylene was placed under the control of arabinose and blue light responsive regulatory systems. The resulting bacteria were capable of accelerating the ripening of tomatoes, kiwifruit, and apples.

引用

页码：935 / 938

页数：4

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