Pathology of urethral fibromuscular system related to parturition-induced stress urinary incontinence and TGF-β1/Smad pathway

被引：26
作者：
Li, Guang-Yong [1 ]
Cui, Wan-Shou [1 ]
Zhou, Feng [1 ]
Gao, Zhe-Zhu [1 ]
Xin, Hua [1 ]
Liu, Tao [1 ]
Li, Wei-Ren [1 ]
Gong, Yan-Qing [1 ]
Bai, Guang-Yi [1 ]
Guo, Ying-Lu [1 ]
Xin, Zhong-Cheng [1 ]
机构：
[1] Peking Univ, Androl Ctr, Hosp 1, Beijing 100034, Peoples R China
来源：
MOLECULAR AND CELLULAR BIOCHEMISTRY | 2012年 / 364卷 / 1-2期
基金：
中国国家自然科学基金;
关键词：
Extracellular matrix; MMP-9; Stress urinary incontinence; Smad2; TGF-beta; 1; SIMULATED BIRTH TRAUMA; PELVIC ORGAN PROLAPSE; CONTINENCE MECHANISM; EXTRACELLULAR-MATRIX; STEM-CELLS; RAT MODEL; PREGNANCY; TISSUE; WOMEN; PROLIFERATION;
D O I：
10.1007/s11010-012-1234-x
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
To study pathological changes of fibromuscular system and the role of TGF-beta 1/Smad pathway in the urethra of a parturition-induced stress urinary incontinence (SUI) rat model. Twenty-eight 8-week-old Sprague-Dawley female rats at gestational day 16 were used and randomized into two groups: sham group and SUI group. After delivery, rats in the SUI group underwent postpartum vaginal balloon dilation and bilateral ovariectomy. 1 month after ovariectomy, urodynamics was assessed. Histological examination (Masson's trichrome stain, picrosirius red stain, Hart's elastin stain, Gordon & Sweet's stain, and immunohistochemical stain) and Western blot were performed on urethral tissues. Both leak point pressure and maximal bladder capacity were significantly decreased in the balloon-injured ovariectomized rats, compared with the sham rats. Muscle was significantly decreased in the urethra of SUI rats compare with sham rats. Collagen I/III and reticular fibers from SUI group were also significantly lower than sham group. Meanwhile, elastic fibers and reticular fibers showed fragmentation and disorganization indicating impairment in the fibromuscular system in SUI rats. TGF-beta 1, MMP-9, and phosphorylated Smad2 (p-Smad2) were expressed significantly higher in SUI than in sham rats. Simulated birth trauma and menopause induced an upregulation of the TGF-beta 1/Smad pathway and impairment of the fibromuscular system in the urethra.
引用
收藏
页码：329 / 335
页数：7
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