Assembly of human immunodeficiency virus type 1 (HIV-1) particles is initiated in the cytoplasm by the formation of a ribonucleoprotein complex comprising the dimeric RNA genome and a small number of viral Gag polyproteins. Genomes are recognized by the nucleocapsid (NC) domains of Gag, which interact with packaging elements believed to be located primarily within the 5'-leader (5'-L) of the viral RNA. Recent studies revealed that the native 5'-L exists as an equilibrium of two conformers, one in which dimer-promoting residues and NC binding sites are sequestered and packaging is attenuated, and one in which these sites are exposed and packaging is promoted. To identify the elements within the dimeric 5'-L that are important for packaging, we generated HIV-1 5'-L RNAs containing mutations and deletions designed to eliminate substructures without perturbing the overall structure of the leader and examined effects of the mutations on RNA dimerization, NC binding, and packaging. Our findings identify a 159-residue RNA packaging signal that possesses dimerization and NC binding properties similar to those of the intact 5'-L and contains elements required for efficient RNA packaging. (C) 2012 Elsevier Ltd. All rights reserved.
机构:
Rockefeller Univ, Aaron Diamond AIDS Res Ctr, New York, NY 10065 USA
Rockefeller Univ, Lab Retrovirol, New York, NY 10065 USARockefeller Univ, Aaron Diamond AIDS Res Ctr, New York, NY 10065 USA
Jouvenet, Nolwenn
;
Bieniasz, Paul D.
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机构:
Rockefeller Univ, Aaron Diamond AIDS Res Ctr, New York, NY 10065 USA
Rockefeller Univ, Lab Retrovirol, New York, NY 10065 USARockefeller Univ, Aaron Diamond AIDS Res Ctr, New York, NY 10065 USA
Bieniasz, Paul D.
;
Simon, Sanford M.
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机构:
Rockefeller Univ, Lab Cellular Biophys, New York, NY 10065 USARockefeller Univ, Aaron Diamond AIDS Res Ctr, New York, NY 10065 USA
机构:
Rockefeller Univ, Aaron Diamond AIDS Res Ctr, New York, NY 10065 USARockefeller Univ, Lab Cellular Biophys, New York, NY 10065 USA
Jouvenet, Nolwenn
;
Simon, Sanford M.
论文数: 0引用数: 0
h-index: 0
机构:
Rockefeller Univ, Lab Cellular Biophys, New York, NY 10065 USARockefeller Univ, Lab Cellular Biophys, New York, NY 10065 USA
Simon, Sanford M.
;
Bieniasz, Paul D.
论文数: 0引用数: 0
h-index: 0
机构:
Rockefeller Univ, Aaron Diamond AIDS Res Ctr, New York, NY 10065 USA
Howard Hughes Med Inst, New York, NY 10016 USARockefeller Univ, Lab Cellular Biophys, New York, NY 10065 USA
机构:Univ Maryland Baltimore Cty, Howard Hughes Med Inst, Baltimore, MD 21250 USA
Lee, BM
;
De Guzman, RN
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机构:Univ Maryland Baltimore Cty, Howard Hughes Med Inst, Baltimore, MD 21250 USA
De Guzman, RN
;
Turner, BG
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机构:Univ Maryland Baltimore Cty, Howard Hughes Med Inst, Baltimore, MD 21250 USA
Turner, BG
;
Tjandra, N
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机构:
Univ Maryland Baltimore Cty, Howard Hughes Med Inst, Baltimore, MD 21250 USAUniv Maryland Baltimore Cty, Howard Hughes Med Inst, Baltimore, MD 21250 USA
Tjandra, N
;
Summers, MF
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机构:Univ Maryland Baltimore Cty, Howard Hughes Med Inst, Baltimore, MD 21250 USA
机构:Univ Maryland Baltimore Cty, Howard Hughes Med Inst, Baltimore, MD 21250 USA
Lu, Kun
;
Heng, Xiao
论文数: 0引用数: 0
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机构:Univ Maryland Baltimore Cty, Howard Hughes Med Inst, Baltimore, MD 21250 USA
Heng, Xiao
;
Summers, Michael F.
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机构:
Univ Maryland Baltimore Cty, Howard Hughes Med Inst, Baltimore, MD 21250 USAUniv Maryland Baltimore Cty, Howard Hughes Med Inst, Baltimore, MD 21250 USA
机构:
Rockefeller Univ, Aaron Diamond AIDS Res Ctr, New York, NY 10065 USA
Rockefeller Univ, Lab Retrovirol, New York, NY 10065 USARockefeller Univ, Aaron Diamond AIDS Res Ctr, New York, NY 10065 USA
Jouvenet, Nolwenn
;
Bieniasz, Paul D.
论文数: 0引用数: 0
h-index: 0
机构:
Rockefeller Univ, Aaron Diamond AIDS Res Ctr, New York, NY 10065 USA
Rockefeller Univ, Lab Retrovirol, New York, NY 10065 USARockefeller Univ, Aaron Diamond AIDS Res Ctr, New York, NY 10065 USA
Bieniasz, Paul D.
;
Simon, Sanford M.
论文数: 0引用数: 0
h-index: 0
机构:
Rockefeller Univ, Lab Cellular Biophys, New York, NY 10065 USARockefeller Univ, Aaron Diamond AIDS Res Ctr, New York, NY 10065 USA
机构:
Rockefeller Univ, Aaron Diamond AIDS Res Ctr, New York, NY 10065 USARockefeller Univ, Lab Cellular Biophys, New York, NY 10065 USA
Jouvenet, Nolwenn
;
Simon, Sanford M.
论文数: 0引用数: 0
h-index: 0
机构:
Rockefeller Univ, Lab Cellular Biophys, New York, NY 10065 USARockefeller Univ, Lab Cellular Biophys, New York, NY 10065 USA
Simon, Sanford M.
;
Bieniasz, Paul D.
论文数: 0引用数: 0
h-index: 0
机构:
Rockefeller Univ, Aaron Diamond AIDS Res Ctr, New York, NY 10065 USA
Howard Hughes Med Inst, New York, NY 10016 USARockefeller Univ, Lab Cellular Biophys, New York, NY 10065 USA
机构:Univ Maryland Baltimore Cty, Howard Hughes Med Inst, Baltimore, MD 21250 USA
Lee, BM
;
De Guzman, RN
论文数: 0引用数: 0
h-index: 0
机构:Univ Maryland Baltimore Cty, Howard Hughes Med Inst, Baltimore, MD 21250 USA
De Guzman, RN
;
Turner, BG
论文数: 0引用数: 0
h-index: 0
机构:Univ Maryland Baltimore Cty, Howard Hughes Med Inst, Baltimore, MD 21250 USA
Turner, BG
;
Tjandra, N
论文数: 0引用数: 0
h-index: 0
机构:
Univ Maryland Baltimore Cty, Howard Hughes Med Inst, Baltimore, MD 21250 USAUniv Maryland Baltimore Cty, Howard Hughes Med Inst, Baltimore, MD 21250 USA
Tjandra, N
;
Summers, MF
论文数: 0引用数: 0
h-index: 0
机构:Univ Maryland Baltimore Cty, Howard Hughes Med Inst, Baltimore, MD 21250 USA
机构:Univ Maryland Baltimore Cty, Howard Hughes Med Inst, Baltimore, MD 21250 USA
Lu, Kun
;
Heng, Xiao
论文数: 0引用数: 0
h-index: 0
机构:Univ Maryland Baltimore Cty, Howard Hughes Med Inst, Baltimore, MD 21250 USA
Heng, Xiao
;
Summers, Michael F.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Maryland Baltimore Cty, Howard Hughes Med Inst, Baltimore, MD 21250 USAUniv Maryland Baltimore Cty, Howard Hughes Med Inst, Baltimore, MD 21250 USA