Prevalence of Theileria infections in goats and sheep in southeastern China

被引：28

作者：

Ge, Yan

Pan, Weiqing

Yin, Hong ^{[1
]}

机构：

[1] Tongji Univ, Dept Immunol & Pathogen Biol, Sch Med, Shanghai 200092, Peoples R China

来源：

VETERINARY PARASITOLOGY | 2012年 / 186卷 / 3-4期

关键词：

Theileria; Goats; Southeastern China; IDENTIFICATION;

D O I：

10.1016/j.vetpar.2011.11.066

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Theileriosis is an important tick-borne hemoprotozoan disease, which can cause severe economic loss in animal husbandry. In this paper, one hundred peripheral blood samples of goats and sheep from southeastern China were examined for the Theileria infection. A region of Theileria 18S rRNA gene was amplified by nested PCR in 26 samples. All the nested PCR amplicons were cloned and sequenced. Alignment analysis has shown that these sequences are highly homologus to each other with identities from 99.2% to 100%. Blast the sequences against NCBI database indicated 99% homology with Theileria sp. China 1 (Theileria luwenshuni). Phylogenetic tree has also shown that the newly identified Theileria are in the same clade with T. luwenshuni. The results have revealed a relatively high prevalence of Theileria in some areas of southeastern China and little genetic diversity in these infections. (C) 2011 Elsevier B.V. All rights reserved.

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页码：466 / 469

页数：4

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