Quercetin Improves Pulmonary Function and Prevents Emphysema Caused by Exposure to Cigarette Smoke in Male Mice

被引:25
作者
da Silva Araujo, Natalia Pereira [1 ,2 ]
de Matos, Natalia Alves [1 ,2 ]
Oliveira, Michel [1 ,2 ]
Farias de Souza, Ana Beatriz [1 ,2 ]
Castro, Thalles de Freitas [1 ,2 ]
Machado-Junior, Pedro Alves [1 ,2 ]
Soares de Souza, Debora Maria [3 ]
Talvani, Andre [3 ]
Cangussu, Silvia Dantas [1 ,2 ]
Alvim de Menezes, Rodrigo Cunha [2 ,4 ]
Bezerra, Frank Silva [1 ,2 ]
机构
[1] Fed Univ Ouro Preto UFOP, Dept Biol Sci, Lab Expt Pathophysiol, BR-35400000 Ouro Preto, Brazil
[2] Fed Univ Ouro Preto UFOP, Ctr Res Biol Sci, BR-35400000 Ouro Preto, Brazil
[3] Fed Univ Ouro Preto UFOP, Inst Exact & Biol Sci, Dept Biol Sci, Lab Immunobiol Inflammat, BR-35400000 Ouro Preto, Brazil
[4] Fed Univ Ouro Preto UFOP, Dept Biol Sci, Lab Cardiovasc Physiol, BR-35400000 Ouro Preto, Brazil
关键词
quercetin; cigarette smoke; oxidative stress; COPD; emphysema; OXIDATIVE STRESS; EXPRESSION; DISEASE; ASTHMA; LUNGS;
D O I
10.3390/antiox11020181
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Chronic obstructive pulmonary disease (COPD) is the major cause of morbidity and mortality worldwide, and cigarette smoke is a key factor in the development of COPD. Thus, the development of effective therapies to prevent the advancement of COPD has become increasingly essential. We hypothesized that quercetin protects lungs in mice exposed to long-term cigarette smoke. Thirty-five C57BL/6 mice were exposed to cigarette smoke (12 cigarettes per day) for 60 days and pretreated with 10 mg/kg/day of quercetin via orogastric gavage. After the experimental protocol, the animals were euthanized and samples were collected for histopathological, antioxidant defense, oxidative stress and inflammatory analysis. The animals exposed to cigarette smoke showed an increase in respiratory rate and hematological parameters, cell influx into the airways, oxidative damage and inflammatory mediators, besides presenting with alterations in the pulmonary histoarchitecture. The animals receiving 10 mg/kg/day of quercetin that were exposed to cigarette smoke presented a reduction in cellular influx, less oxidative damage, reduction in cytokine levels, improvement in the histological pattern and improvement in pulmonary emphysema compared to the group that was only exposed to cigarette smoke. These results suggest that quercetin may be an agent in preventing pulmonary emphysema induced by cigarette smoke.
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页数:13
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