Reconstitution of a functional core polycomb repressive complex

被引：300

作者：

Francis, NJ

Saurin, AJ

Shao, ZH

Kingston, RE ^{[1
]}

机构：

[1] Massachusetts Gen Hosp, Dept Mol Biol, Boston, MA 02114 USA

[2] Harvard Univ, Sch Med, Dept Genet, Boston, MA 02115 USA

来源：

MOLECULAR CELL | 2001年 / 8卷 / 03期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/S1097-2765(01)00316-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The opposing actions of polycomb (PcG) and trithorax group (trxG) gene products maintain essential gene expression patterns during Drosophila development. PcG proteins are thought to establish repressive chromatin structures, but the mechanisms by which this occurs are not known. Polycomb repressive complex 1 (PRC1) contains several PcG proteins and inhibits chromatin remodeling by trxG-related SWI/SNF complexes. We have defined a functional core of PRC1 by reconstituting a stable complex using four recombinant PcG proteins. One subunit, PSC, can also inhibit chromatin remodeling on its own. These PcG proteins create a chromatin structure that has normal nucleosome organization and is accessible to nucleases but excludes hSWI/SNF.

引用

页码：545 / 556

页数：12

共 46 条

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