Single-walled carbon nanotubes covalently functionalized with polytyrosine: A new material for the development of NADH-based biosensors

We report for the first time the use of single-walled carbon nanotubes (SWCNT) covalently functionalized with polytyrosine (Polytyr) (SWCNT-Polytyr) as a new electrode material for the development of nicotinamide adenine dinucleotide (NADH)-based biosensors. The oxidation of glassy carbon electrodes (GCE) modified with SWCNT-Polytyr at potentials high enough to oxidize the tyrosine residues have allowed the electrooxidation of NADH at low potentials due to the catalytic activity of the quinones generated from the primary oxidation of tyrosine without any additional redox mediator. The amperometric detection of NADH at 0.200 V showed a sensitivity of (217 +/- 3) mu A mM(-1) cm(-2) and a detection limit of 7.9 nM. The excellent electrocatalytic activity of SWCNT-Polytyr towards NADH oxidation has also made possible the development of a sensitive ethanol biosensor through the immobilization of alcohol dehydrogenase (ADH) via Nafion entrapment, with excellent analytical characteristics (sensitivity of (5.8 +/- 0.1) mu A mM(-1) cm(-2), detection limit of 0.67 mu M) and very successful application for the quantification of ethanol in different commercial beverages. (C) 2016 Elsevier B.V. All rights reserved.